Test Overview
Test Methodology

Chemiluminescence

Test Usage

Diagnosis of vitamin D deficiency, differential diagnosis of causes of rickets and osteomalacia, monitoring vitamin D replacement therapy, and diagnosis of hypervitaminosis D.

Reference Range *

Optimal: 25 - 100 ng/mL; Deficiency: <10 ng/mL; Toxicity: >150 ng/mL.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Daily, 24 hours
Analytic Time

4 hours

Soft Order Code
25HD
MiChart Code
Vitamin D 25 Hydroxy (25HD)
Synonyms
  • 25-hydroxy Vitamin D
  • Calcidiol
  • Vitamin D 25-OH
  • Vitamin D2
  • Vitamin D3
  • 25HD
  • 25-Hydroxyvitamin D, Total
Laboratory
Chemical Pathology
Section
Automation
Specimen Requirements
Collection Instructions

Collect blood in red top or SST tube. Centrifuge, aliquot serum into a plastic vial, and refrigerate up to 6 days or freeze for longer storage.

Alternate Specimen
Red top, lavender top (EDTA), and green top (sodium heparin) tubes are acceptable.
Yellow Top Tube
Normal Volume
0.5 mL serum
Minimum Volume
0.25 mL serum
Billing
CPT Code
82306
Fee Code
32032
LOINC
1989-3
Resources