Test Overview
Chemiluminescence
Diagnosis of vitamin D deficiency, differential diagnosis of causes of rickets and osteomalacia, monitoring vitamin D replacement therapy, and diagnosis of hypervitaminosis D.
Optimal: 25 - 100 ng/mL; Deficiency: <10 ng/mL; Toxicity: >150 ng/mL.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Samples containing fluorescein may produce falsely elevated values with this assay. Samples should be obtained at least 48 to 72 hours post treatment.
Test Details
4 hours
- 25-hydroxy Vitamin D
- Calcidiol
- Vitamin D 25-OH
- Vitamin D2
- Vitamin D3
- 25-Hydroxyvitamin D, Total
Specimen Requirements
Collect blood in red top or SST tube. Centrifuge, aliquot serum into a plastic vial, and refrigerate up to 6 days or freeze for longer storage.
Red top, lavender top (EDTA), and green top (sodium heparin) tubes are acceptable.
0.5 mL serum
0.25 mL serum