Test Overview
Test Methodology

Diasorin Chemiluminescent Immunoassay

Test Usage

Differential diagnosis of hypercalcemia. Levels are decreased in chronic renal failure and hypoparathyroidism and increased in sarcoidosis.

Reference Range *

18 - 78 pg/mL

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Varies; test performed twice per week
Soft Order Code
MiChart Code
Vitamin D 1,25 Dihydroxy (DHVD)
  • 1,25 Dihydro Cholecalciferol
  • 1,25 Dihydrocholecalciferol
  • 1,25-dihydroxy Vitamin D
  • Calcitriol
  • DHVD
Chemical Pathology
Special Chemistry
Specimen Requirements
Collection Instructions

Collect specimen in SST (preferred) or red top tube from a fasting patient (a fasting specimen is recommended, but not required). Centrifuge, aliquot serum into a plastic vial, and refrigerate up to 6 days or freeze for longer storage.

Special Handling

Fasting specimen.

Normal Volume
1.0 mL of serum
Minimum Volume
0.5 mL of serum
Additional Information

While 1,25-dihydroxy vitamin D is the most potent vitamin D metabolite, levels of the 25-OH forms of vitamin D more accurately reflect the body's vitamin D stores. Consequently, Vitamin D 25-hydroxy is the preferred initial test for assessing vitamin D status. However, in the presence of renal disease, 1,25-dihydroxy vitamin D levels may be needed to adequately assess vitamin D status.

CPT Code
Fee Code