Test Overview
Test Methodology

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Test Usage

Assessment of thiamin deficiency. Thiamin measurement is appropriate in patients with behavioral changes, eye signs, gait disturbances, delirium, and encephalopathy; or in patients with questionable nutritional status, especially those who appear at risk and who also are being given insulin for hyperglycemia.

Reference Range *

70 - 180 nmol/L

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Vitamin supplementation and nonfasting specimens may result in elevated thiamine diphosphate concentrations.

Test Details
Days Set Up
Monday - Thursday
Analytic Time

4-7 days

Soft Order Code
MiChart Code
Vitamin B1, Whole Blood
  • Thiamin (Vitamin B1), WB
  • B1, Whole Blood
  • B1
  • Thiamine, Whole Blood
  • Thiamine
  • TDP
  • Vitamin
Reference Laboratory
Mayo TDP (85753)
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in a lavender top tube (EDTA) following an overnight (12 hour) fast. Invert 8-10 times to mix blood, pour whole blood into an plastic vial, and freeze. Protect specimen from light.DO NOT SPIN

Special Handling

Fasting specimen. Protect specimen from light. Aliquot (pour) whole blood to amber PFL tube before freezing.DO NOT SPIN

Normal Volume
4 mL whole blood
Minimum Volume
0.5 mL whole blood
Storage Temperature
Strict frozen
Rejection Criteria
Specimen not protected from light. Not frozen as whole blood in amber PFL tube.
Specimen received as plasma at Mayo. DO NOT SPIN
Additional Information

Test sent to Mayo Medical Laboratories.

CPT Code
Fee Code