2 - 7 days
Uridine Diphosphate Glucuronyltransferase 1A1 Promoter Genotyping
UGT1A1 Promoter Genotyping PCR
Irinotecan Sensitivity Analysis
Looking to order a test?
We’ve provided helpful links to make ordering easy.Find a Requisition
All specimens should be accompanied by a requisition.Submitting Specimens
Learn about how to properly label and where to ship specimens.Order Kits and Supplies
MLabs provides all the supplies necessary for the collection of specimens.Test FAQ
Visit our provider FAQ and learn about common questions to ordering tests.
Polymerase Chain Reaction (PCR) with capillary electrophoresis
Irinotecan, also known as Camptosar® or CPT-11, is an anti-neoplastic agent used to treat colorectal cancers. Uridine diphosphate glucuronyltransferase 1A1 (UGT1A1) is responsible for the metabolic inactivation of SN-38 (active form of Irinotecan) via glucuronidation. Individuals with low levels of UGT1A1 activity are at increased risk for toxicity following Irinotecan administration, due to the presence of elevated levels of unconjugated SN-38. Decreased UGT1A1 activity can result from mutations in the coding sequence of the UGT1A1 gene, which alter the structure of the encoded protein, or from decreased expression of the normal protein product. A common cause of decreased expression levels of UGT1A1 is a variation in the number of TA repeats in the TATA box of the UGT1A1 gene promoter. The (TA)6 (i.e., 6 TA repeats) version of the promoter is considered to be the "normal" or wild type. Compared to the (TA)6 promoter, the (TA)7 version has a decreased activity, leading to the production of lower levels of UGT1A1 transcripts. To date, alleles containing 5, 6, 7, and 8 TA repeats have been identified. Several clinical studies have shown that individuals who are homozygous, and potentially those who are heterozygous, for the UGT1A1 (TA)7 promoter allele (UGT1A1*28) are at increased risk for the development of a significant adverse response to the standard dose of Irinotecan. Therefore, in July of 2005, the FDA altered the package labeling of Irinotecan to suggest a lower starting dose of Irinotecan for patients known to be homozygous for the UGT1A1 *28 (i.e., 7 TA repeat) allele.
Interpretive report provided.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Collect blood in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection.