Test Overview
Test Methodology

Fluorescence In Situ Hybridization (FISH)

Test Usage

This test detects rearrangements involving the TFE3 gene region (Xp11.2) via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded tissue specimens. FISH is performed using a dual-color break-apart probe (Empire Genomics) to qualitatively assess for the presence of TFE3 translocations that are associated with certain subtypes of renal cell carcinoma. Testing for TFE3 gene rearrangement may aid in the diagnosis and classification of renal tumors.

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

This test detects rearrangements involving TFE3, however it will not identify the translocation partner.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:00pm
Analytic Time

10-14 days

Soft Order Code
MiChart Code
TFE3 Rearrangement by FISH
  • CGTF3
  • Renal Cell Carcinoma
  • FFPE FISH for TFE3
  • TFE3 gene region Xp11.2
Specimen Requirements
Collection Instructions

Submit a formalin-fixed, paraffin block of the renal tumor tissue (with matched benign renal tissue if available); store at room temperature.

Additional Information

By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws.

CPT Code
88271, 88275
Fee Code
EA042, EA043