Reverse transcription followed by Polymerase Chain Reaction (PCR) with detection by capillary electrophoresis.
Synovial sarcoma is characterized by the t(X;18)(p11;q11) translocation which occurs in over 90% of cases regardless of histologic subtype. This rearrangement leads to fusion of the SYT (SS18) gene on chromosome 18 with one of several closely related SSX genes located on the X chromosome. Approximately two-thirds of fusions are SYT/SSX1 and one-third SYT/SSX2. Other fusions are rare. Because of their high specificity and prevalence, SYT/SSX fusion transcripts are highly sensitive diagnostic markers for synovial sarcoma. Testing can aid in the differential diagnosis of synovial sarcoma, especially in tumors with unusual histologic or clinical presentations. This test qualitatively detects the SYT/SSX1 and SYT/SSX2 fusion transcripts in fresh/frozen or formalin-fixed paraffin embedded tissues.
Interpretive report provided. The specific fusion transcript type is reported with a positive test result.
2 - 7 days
- Synovial Sarcoma Translocation Detection
- SYTSSX Translocation Assay
- SSX Xp11
- SYT 18q11
- SYTSSX Translocation
- t(X;18) Translocation
A formalin-fixed, paraffin-embedded tissue block containing an area with a high percentage of neoplastic cells (for micro-/macro-dissection) is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.
By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.