Test Overview
Test Methodology

SYPNB: Multiplex Flow Immunoassay
RPRS, RRPRQ: Flocculation/Agglutination
RTPPA: Particle Agglutination

Test Usage

An aid in the diagnosis of active syphilis (Treponema pallidum) infection and routine prenatal screening.

Reference Range *


* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Despite active syphilis, serologic tests may be negative in severely immunosuppressed patients such as those with AIDS. In very early cases of primary syphilis, serology tests for syphilis may be negative. In cases of untreated, late/latent syphilis, the result of RPR may be negative. However, the syphilis screening test (MFI) and TP-PA should be positive.

Test Details
Days Set Up
Monday - Friday, Sunday
Analytic Time

2 - 5 days

Soft Order Code
Reference Laboratory
Mayo SYPH1
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in an SST or Red top tube. Centrifuge, aliquot serum into plastic vial and freeze.

Normal Volume
1.5 mL serum
Minimum Volume
1 mL serum
Additional Information

If the results for IgG are positive, a Rapid Plasma Reagin (RPR) assay will be performed automatically at an additional charge. If the RPR is positive, an RPR Titer will be performed at an additional charge. If the RPR is negative, Syphilis Antibody TP-PA will be performed at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. Test sent to Mayo Medical Laboratories.

CPT Code
Fee Code
Reflex CPT
86592, 86593, 86780