Update Type: New Tests
Test Updated: 11/27/2024
Test Overview
Test Methodology

Multiplex bead Immunoassay

Test Usage

Syphilis reverse algorithm is used to screen patients for syphilis. Positive or equivocal result will reflex to RPR and TPPA, if indicated to help differentiate between active infection, past infection, no infection or false positive total antibody. All results must be reviewed before making a clinical decision.

Reference Range *

Nonreactive

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

A nonreactive result does not exclude the possibility of exposure to or infection with T. pallidum. Antibodies may be at low or undetectable levels in incubating or early primary disease and in some clinical conditions.
Detection of treponemal antibodies may indicate recent, past or successfully treated syphilis infections and therefore cannot be used to differentiate between active and cured cases.
A patient with a Syphilis Total reactive result will usually remain reactive for life, and therefore, antibody indices cannot be used to determine responses to therapy. In these cases, an RPR should be used to monitor the patient.

Test Details
Days Set Up
M-F
Analytic Time

24 hours

Soft Order Code
SYPH
MiChart Code
Syphilis Screening, Reverse Algorithm
Laboratory
Chemical Pathology
Section
Immunopathology
Specimen Requirements
Collection Instructions

Collect SST and send refrigerated to main hospital specimen processing

Alternate Specimen
red top, EDTA or heparin
Contraindications
Contaminated, icteric, lipemic, hemolyzed or heat inactivated sera my cause erroneous results and should be avoided.
Normal Volume
1.0 ml
Minimum Volume
0.5 ml
Storage Temperature
Room temperature (25C) up to 3 days; refrigerated (2-8C) up to 7 days. Longer storage, keep at -20C or colder
Rejection Criteria
Avoid contaminated, icteric, lipemic, hemolyzed or heat inactivated sera.
Additional Information

All reactive and equivocal results will reflex to an RPR and TPPA, if indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

Billing
CPT Code
86780
Fee Code
KA057
Reflex CPT
RPR 86592, RPRQN 86593, TPPA 86780
Reflex Fee Code
RPR 21922, RPRQN 21923, TPPA KA050
LOINC
47236-5