Test Overview
Test Methodology

Direct Immunofluorescence

Test Usage

To determine the presence of Treponema pallidum in a syphilitic chancre.

Reference Range *

Treponema pallidum not found.

Test Limitations

Failure to demonstrate the presence of Treponema pallidum does not indicate its absence in the lesion.

Test Details
Days Set Up
Monday - Friday, 7:30am - 5:30pm
Analytic Time

Up to one week

Soft Order Code
  • Spirochete Smear
  • Treponema pallidum Darkfield
  • Darkfield, Treponema pallidum
  • Fluorescent Antibody Darkfield Test
  • Treponema pallidum Darkfield Exam
  • Syphilis Darkfield
Reference Laboratory
Special Testing
Specimen Requirements
Collection Instructions

Call the Michigan Department of Health & Human Services at 517-335-8080 and request kit: Fluorescent Darkfield for Treponema pallidum, Unit number 10, with Blanks FB10 and FB8. Air dry the slides for 20 minutes to inactivate the treponemes and render them non-infectious before transporting. Place slides in the cardboard book-type mailer; store at room temperature. Fill in the information requested on the form labeled "Examination for Treponema pallidum", using a pen with black ink or typewriter with black ribbon.

Additional Information

Test sent to Michigan Department of Health & Human Services (MDHHS); although there is no charge for testing, MLabs charges a handling fee for processing and forwarding specimens to MDHHS.

CPT Code
Fee Code
NY State Approved