Days Set Up
Monday - Friday, 7:30am - 5:30pm
Analytic Time

Up to one week

Soft Order Code

Test Updated:


Spirochete Smear
Treponema pallidum Darkfield
Darkfield, Treponema pallidum
Fluorescent Antibody Darkfield Test
Treponema pallidum Darkfield Exam
Syphilis Darkfield

Looking to order a test?

We’ve provided helpful links to make ordering easy.

Find a Requisition

All specimens should be accompanied by a requisition.

Submitting Specimens

Learn about how to properly label and where to ship specimens.

Order Kits and Supplies

MLabs provides all the supplies necessary for the collection of specimens.

Test FAQ

Visit our provider FAQ and learn about common questions to ordering tests.

Test Overview

Test Methodology

Direct Immunofluorescence

Test Usage

To determine the presence of Treponema pallidum in a syphilitic chancre.

Reference Range

Treponema pallidum not found.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Offsite

Call the Michigan Department of Health & Human Services at 517-335-8080 and request kit: Fluorescent Darkfield for Treponema pallidum, Unit number 10, with Blanks FB10 and FB8. Air dry the slides for 20 minutes to inactivate the treponemes and render them non-infectious before transporting. Place slides in the cardboard book-type mailer; store at room temperature. Fill in the information requested on the form labeled "Examination for Treponema pallidum", using a pen with black ink or typewriter with black ribbon.

Minimum Volume

Billing Information

CPT Code
Pro Fee Code

Have More Questions?

Explore our helpful billing resources:

Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.