Update Type: Test Down or Delayed
Test Updated: 05/17/2023
Test Overview
Test Methodology

Particle agglutination

Test Usage

Aids in the diagnosis of syphilis by confirming presence of Treponema pallidum antibodies.

Reference Range *


* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

TPPA may be reactive in persons from areas where yaws and pinta is or was endemic. Samples from patients with HIV, Leprosy, Toxoplasmosis, H. Pylori, and drug addiction may react with test particles causing false-positive or inconclusive results.

Test Details
Days Set Up
Tuesday, Friday
Analytic Time

8 hours

Soft Order Code
MiChart Code
Syphilis Antibody, Treponema pallidum Particle Agglutination, Serum
Chemical Pathology
Specimen Requirements
Collection Instructions

Collect specimen in SST tube or EDTA, if necesary. Centrifuge, aliquot serum into plastic vial and refrigerate. Store at 2-8C up to 5 days. Freeze serum for longer storage.

Normal Volume
1.5 mL serum
1.5 mL plasma
Minimum Volume
1.0 mL serum
1.0 mL plasma
Storage Temperature
Rejection Criteria
Samples frozen and thawed more than once.
Additional Information

The identification of Treponema pallidum antibodies aids in the diagnosis of syphilis caused by the microorganisms belonging to the genus Treponema and provides epidemiological information on syphilis. A reactive treponemal test indicates past or present infection and usually remains positive for life. A non-reactive result indicates no past or present infection, but may occur during incubation-stage syphilis.

CPT Code
Fee Code