Test Overview
Molecular Typing (SSO) and Solid Phase Assay
At the UMHS Histocompatibility Laboratory potential recipients are typed for HLA-A, B, C, DRB1,3,4,5, DQB1, DQA1 loci by medium/low resolution SSO molecular methods. Each recipient is screened to detect preformed anti-HLA antibodies. Patients may develop these antibodies in case of previous sensitization events like transfusion, pregnancies or previous transplant. When these antibodies are detected the Panel Reactive Antibody (PRA%) value is assigned and the anti-HLA antibody specificity is defined by Solid Phase Single Antigen assays. PRA% values are updated with a new sample every 3 months. For patients listed for transplant with a deceased donor the Histocompatibility Laboratory will updated PRA% and the Unacceptable Antigens list in the UNOS database every 3 months. Every month a fresh serum sample is mailed to the Organ Procurement Organization (OPO). This serum sample and the historic higher PRA% sample (peak) will be used for Flow Cytometric Crossmatch with matched deceased donors.
Interpretive report provided.
Test Details
5 days
- HLA Antibody Screening
- HLA Typing Class I and II
- Percent Reactive Antibody (PRA)
- New Patient, Solid Organ Tx
- SONP
- HLA REPORT
- HLLR
- HLA Antibody Specificity Class II
- HLA Class II Low Resolution
- HLA Class I Low Resolution
- TTYPE
- TISSUE TYPING
- HLA Antibody Specificity Class I
- HLAS
Specimen Requirements
Collect specimens in sufficient yellow top (ACD) solution A tube AND SST tubes. Send intact specimens at room temperature. Do not refrigerate or freeze. Specify type of organ (Kidney, Heart, Lung, Liver, or Pancreas).
Red top or SST tube is always required for antibody testing.
Additional Information
Test includes HLA Typing Class I & II and Antibody Specificity Class I & II.