Update Type: Miscellaneous
Test Updated: 11/22/2023
Test Overview
Test Methodology

Molecular Typing (SSO) and Solid Phase Assay

Test Usage

At the UMHS Histocompatibility Laboratory potential recipients are typed for HLA-A, B, C, DRB1,3,4,5, DQB1, DQA1, DPB1, DPA1 loci by medium/low resolution SSO molecular methods. Each recipient is screened to detect preformed anti-HLA antibodies. Patients may develop these antibodies in case of previous sensitization events like transfusion, pregnancies or previous transplant. When these antibodies are detected the Panel Reactive Antibody (PRA%) value is assigned and the anti-HLA antibody specificity is defined by Solid Phase Single Antigen assays. PRA% values are updated with a new sample every 3 months. For patients listed for transplant with a deceased donor the Histocompatibility Laboratory will updated PRA% and the Unacceptable Antigens list in the UNOS database every 3 months. Every month a fresh serum sample is mailed to the Organ Procurement Organization (OPO). This serum sample and the historic higher PRA% sample (peak) will be used for Flow Cytometric Crossmatch with matched deceased donors.

HLA Typing is genetic typing, and the data will not change. Typing cannot be performed repeatedly for patients or donors.

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm
Analytic Time

5 days

Soft Order Code
MiChart Code
Lab 7588 New Patient (HLA Typing I & 2, Antibody Screening PRA)
  • HLA Antibody Screening
  • HLA Typing Class I and II
  • Percent Reactive Antibody (PRA)
  • New Patient, Solid Organ Tx
  • SONP
  • HLLR
  • HLA Antibody Specificity Class II
  • HLA Class II Low Resolution
  • HLA Class I Low Resolution
  • HLA Antibody Specificity Class I
  • HLAS
Specimen Requirements

Collect specimens in sufficient yellow top (ACD) solution A tube AND SST tubes. Send intact specimens at room temperature. Do not refrigerate or freeze. Specify type of organ (Kidney, Heart, Lung, Liver, or Pancreas).

HLLRD: 10 mL Yellow top (ACD) solution A tube
HLAS: 5 mL SST tube

Alternate Specimen
For typing (HLLRD) ACD (Solution B), lavender EDTA
For antibody testing (HLAS) Red top or SST tube is always required for antibody testing.
Normal Volume
HLLRD: 20mL Yellow top (ACD) solution A tube
HLAS: 5mL SST tube
Minimum Volume
HLLRD: 10 mL Yellow top (ACD) solution A tube
HLAS: 2 mL SST tube
Additional Information

Test includes HLA Typing Class I & II and Antibody Specificity Class I & II.

CPT Code
81370, 81376 x3, 86832, 86833
Fee Code
OA006, OA007 x3, 37962, 37963