Molecular Typing (SSO) and Solid Phase Assay
At the UMHS Histocompatibility Laboratory potential recipients are typed for HLA-A, B, C, DRB1,3,4,5, DQB1, DQA1 loci by medium/low resolution SSO molecular methods. Each recipient is screened to detect preformed anti-HLA antibodies. Patients may develop these antibodies in case of previous sensitization events like transfusion, pregnancies or previous transplant. When these antibodies are detected the Panel Reactive Antibody (PRA%) value is assigned and the anti-HLA antibody specificity is defined by Solid Phase Single Antigen assays. PRA% values are updated with a new sample every 3 months. For patients listed for transplant with a deceased donor the Histocompatibility Laboratory will updated PRA% and the Unacceptable Antigens list in the UNOS database every 3 months. Every month a fresh serum sample is mailed to the Organ Procurement Organization (OPO). This serum sample and the historic higher PRA% sample (peak) will be used for Flow Cytometric Crossmatch with matched deceased donors.
Interpretive report provided.
- HLA Antibody Screening
- HLA Typing Class I and II
- Percent Reactive Antibody (PRA)
- New Patient, Solid Organ Tx
- HLA REPORT
- HLA Antibody Specificity Class II
- HLA Class II Low Resolution
- HLA Class I Low Resolution
- TISSUE TYPING
- HLA Antibody Specificity Class I
Collect specimens in sufficient yellow top (ACD) solution A tube AND SST tubes. Send intact specimens at room temperature. Do not refrigerate or freeze. Specify type of organ (Kidney, Heart, Lung, Liver, or Pancreas).
Test includes HLA Typing Class I & II and Antibody Specificity Class I & II.