Test Overview
Test Methodology

Molecular Typing (SSO) and Solid Phase Assay

Test Usage

At the UMHS Histocompatibility Laboratory potential recipients are typed for HLA-A, B, C, DRB1,3,4,5, DQB1, DQA1 loci by medium/low resolution SSO molecular methods. Each recipient is screened to detect preformed anti-HLA antibodies. Patients may develop these antibodies in case of previous sensitization events like transfusion, pregnancies or previous transplant. When these antibodies are detected the Panel Reactive Antibody (PRA%) value is assigned and the anti-HLA antibody specificity is defined by Solid Phase Single Antigen assays. PRA% values are updated with a new sample every 3 months. For patients listed for transplant with a deceased donor the Histocompatibility Laboratory will updated PRA% and the Unacceptable Antigens list in the UNOS database every 3 months. Every month a fresh serum sample is mailed to the Organ Procurement Organization (OPO). This serum sample and the historic higher PRA% sample (peak) will be used for Flow Cytometric Crossmatch with matched deceased donors.

Reference Range *

Interpretive report provided.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm
Analytic Time

5 days

Soft Order Code
MiChart Code
Lab 7588 New Patient (HLA Typing I & 2, Antibody Screening PRA)
  • HLA Antibody Screening
  • HLA Typing Class I and II
  • Percent Reactive Antibody (PRA)
  • New Patient, Solid Organ Tx
  • SONP
  • HLLR
  • HLA Antibody Specificity Class II
  • HLA Class II Low Resolution
  • HLA Class I Low Resolution
  • HLA Antibody Specificity Class I
  • HLAS
Specimen Requirements

Collect specimens in sufficient yellow top (ACD) solution A tube AND SST tubes. Send intact specimens at room temperature. Do not refrigerate or freeze. Specify type of organ (Kidney, Heart, Lung, Liver, or Pancreas).

Alternate Specimen
For typing (HLLR) ACD (Solution B), lavender EDTA
Red top or SST tube is always required for antibody testing.
Normal Volume
20 mL ACD (solution A) whole blood AND 5 mL serum
Minimum Volume
10 mL ACD (solution A) whole blood AND 2 mL serum
Additional Information

Test includes HLA Typing Class I & II and Antibody Specificity Class I & II.

CPT Code
81370, 81376 x3, 86832, 86833
Fee Code
OA006, OA007 x3, 37962, 37963
NY State Approved