Test Overview
Molecular Typing (Polymerase Chain Reaction with SSO)
This assay is recommended for highly sensitized recipients previously exposed to a vascularized tissue like a previous transplant or vascular reconstruction surgeries.
Test Details
5 days
- TTYPE
- TISSUE TYPING
- HLA REPORT
- HLA Class I One Locus (Low Resolution)
- MICAT
- MICA Typing
- MICA Antigen Typing
- HLA Class I One Locus (Low Resolution)
Specimen Requirements
Collect specimen in sufficient yellow top (ACD) solution A tubes. Send intact specimen at room temperature. Do not refrigerate or freeze.
Additional Information
Major histocompatibility complex class I-related chain A (MICA) is a polymorphic gene family with more than 70 alleles described, located in the MHC Class I region on chromosome 6. This gen encodes a cell surface glycoprotein molecule that may become a target for both cellular and humoral immune responses. MICA antigens are able to induce the synthesis of alloantibodies in transplant recipients.These antibodies have been found in association with irreversible allograft rejection, an increased frequency of acute rejection episodes, and a significantly lower graft survival. The expression MICA is limited to fibroblast, epithelial and endothelial cells. Resting lymphocytes do not express MICA then antibodies against MICA are not detected by the regular flow cytometric crossmatch. Our laboratory genotype recipient and donor for MICA with the same SSO molecular technology utilized for HLA genotyping. For the detection and characterization of antibodies against MICA our laboratory uses solid phase single antigen technology.