Test Overview
Test Methodology

Molecular Typing (Polymerase Chain Reaction with SSO)

Test Usage

This assay is recommended for highly sensitized recipients previously exposed to a vascularized tissue like a previous transplant or vascular reconstruction surgeries.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm
Analytic Time

5 days

Soft Order Code
MICAT
MiChart Code
MICA Typing
Synonyms
  • TTYPE
  • TISSUE TYPING
  • HLA REPORT
  • HLA Class I One Locus (Low Resolution)
  • MICAT
  • MICA Typing
  • MICA Antigen Typing
  • HLA Class I One Locus (Low Resolution)
Laboratory
Histocompatibility/Immunogenetics
Section
Histocompatibility
Specimen Requirements
Collection

Collect specimen in sufficient yellow top (ACD) solution A tubes. Send intact specimen at room temperature. Do not refrigerate or freeze.

Alternate Specimen
Yellow top ACD (Solution B) tube, lavender top EDTA tube, Green top (sodium or lithium heparin) tubes
Normal Volume
10 mL ACD (solution A) whole blood
Minimum Volume
5 mL ACD (solution A) whole blood
Additional Information

Major histocompatibility complex class I-related chain A (MICA) is a polymorphic gene family with more than 70 alleles described, located in the MHC Class I region on chromosome 6. This gen encodes a cell surface glycoprotein molecule that may become a target for both cellular and humoral immune responses. MICA antigens are able to induce the synthesis of alloantibodies in transplant recipients.These antibodies have been found in association with irreversible allograft rejection, an increased frequency of acute rejection episodes, and a significantly lower graft survival. The expression MICA is limited to fibroblast, epithelial and endothelial cells. Resting lymphocytes do not express MICA then antibodies against MICA are not detected by the regular flow cytometric crossmatch. Our laboratory genotype recipient and donor for MICA with the same SSO molecular technology utilized for HLA genotyping. For the detection and characterization of antibodies against MICA our laboratory uses solid phase single antigen technology.

Billing
CPT Code
81373
Fee Code
37924
NY State Approved
No
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