Test Overview
Test Methodology

Enzyme Immunoassay (EIA)

Test Usage

Detection of presence of shiga toxin 1 and 2.

Reference Range *

No shiga toxin detected.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

The limits of detection of SLT-1 and SLT-2 are approximately 7 pg/well and 15 pg/well, respectively. This test detects the presence of shiga toxin of enterohemorrhagic Escherichia coli and Shigella dysenteriae type 1 only. For detection of other enteric pathogens (Salmonella, Shigella, Campylobacter, Aeromonas, Plesiomonas, Yersinia, Vibrio), see "Stool, Aerobic Culture". A positive result does not preclude the presence of other infectious organisms.

Test Details
Days Set Up
Analytic Time

24 hours

Soft Order Code
MiChart Code
Shiga-like Toxin (E. coli 0157), Stool
  • E coli O157
  • E. coli O157
  • Enterohemorrhagic E coli
  • Enterohemorrhagic E. coli
  • Escherichia coli O157
  • Hemolytic Uremic Syndrome (HUS)
  • Shiga toxin
  • Shiga toxin 1 and 2
  • SLT (Shiga-Like Toxin)
  • Verotoxin
  • SLT
Specimen Requirements
Collection Instructions

Collect fresh random stool or rectal swab. Add stool specimen to transport until liquid reaches fill line. Emulsify specimen thoroughly in transport fluid. Send stool in transport within 72 hrs of collection or rectal swab in aerobic transport within 24 hrs. Do not refrigerate or incubate. Bacterial isolates are also acceptable. If Hemolytic Uremic Syndrome is suspected or stool is bloody, indicate this on the requisition. No more than 1 specimen per day will be accepted. Indicate specimen source and collection date/time.

Normal Volume
2 - 5 mL stool or 1 swab (ESwab)
Minimum Volume
2 mL stool or 1 swab (ESwab)
Storage Temperature
Refrigerate or room temperature
Rejection Criteria
Stool in C&S transport greater than 72 hours old
Stool in ESwab transport over 24 hours old
Fresh stool over 2 hours old
CPT Code
Fee Code