Update Type: Test Resumed
Test Updated: 08/09/2023
Test Overview
Test Methodology

Direct immunofluorescence using flow cytometric techniques.

Test Usage

Evaluation of possible severe combined immunodeficiency. Testing is performed only on infants identified by State of Michigan TREC study as potential SCIDS patients. A full lymphoid cell panel is run, including B and T cell, T subsets, T-memory and naive subsets, and natural killer cell quantitation. Markers include:

  • CD3+
    CD3+CD4+
    CD3+CD8+
    CD3-CD16/56+
    CD19+
    CD3+CD4+CD45RA+
    CD3+CD4+CD45RO+
    CD3+CD8+CD45RA+
    CD3+CD8+CD45RO+
    Calculated CD4:CD8 ratio (T4:T8 ratio)
Reference Range *


*Pediatric Reference Ranges from Journal of Allergy and Clinical Immunology, vol. 112, No. 5, 11/2003 and studies performed at Michigan Medicine Flow Cytometry Laboratory. Absolute ranges are calculated utilizing absolute lymphocyte count ranges for the same age groups provided by the hematology laboratory.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Requires an adequate number of viable cells for analysis. Improper collection or transport may result in decreased cell viability and cancellation of testing.Specimen collection should be arranged so that specimen will be received Monday-Friday, 8:00 am - 4:30 pm. Refrigeration of specimens may result in the deterioration of surface markers.

  • Absolute counts CANNOT be reported without accompanying CBCD.
Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm; Saturday, 8:00am - 12:00noon
Analytic Time

24 - 48 hours

Soft Order Code
SCIDS
MiChart Code
SCID panel: T,B,NK Quant (CD3,4,8,19,16/56) with 45RA/RO. Restricted to pat
Synonyms
  • SCID (Severe Combined Immunodeficiency Disorder)
  • Immunodeficiencies Profile, SCID
  • Newborn Screen
  • SCIDS PANEL
  • SCIDS
  • SCIDT
  • SCID Panel Shadow
Laboratory
Hematopathology
Section
Hematology
Specimen Requirements
Collection Instructions

Testing performed only for University of Michigan registered patients.

Alternate Specimen
Lavender EDTA and yellow ACD A or B Vacutainer® tubes are acceptable, but not preferred for the flow testing.
Normal Volume
Whole blood: 3 ml added to each tube
Minimum Volume
Whole blood: 1 ml added to each tube tube
Rejection Criteria
Green top lithium tubes are not acceptable.
Billing
CPT Code
86355, 86359, 86360, 86357, 86356 x4
Fee Code
23043, 23044, 23045, 23047, MA001 x4