Update Type: Test Code Change
Test Updated: 08/03/2022
Test Overview
Test Methodology

LC-MS/MS

Test Usage

Detection of heparin-dependent platelet antibodies.

Reference Range *

Negative. A positive result requires >20% release of serotonin with low dose heparin and

Test Limitations

The presence of "non-drug" antibodies reactive with platelets (HLA Class I, autoantibodies, platelet-specific antibodies, etc.) in the patient’s serum can induce heparin independent release of serotonin in the SRA.

Test Details
Days Set Up
Monday - Friday
Analytic Time

2 - 4 days

Soft Order Code
SRAU
MiChart Code
Heparin Induced Serotonin Release Assay
Synonyms
  • Heparin Antibodies
  • Heparin Associated Thrombocytopenia and Thrombosis
  • Heparin Associated Thrombosis (HAT)
  • Heparin Dependent
  • Heparin Dependent Antiplatelet Antibodies
  • Heparin Induced Serotonin Release Assay
  • Heparin Induced Thrombosis (HIT)
  • Heparin-Associated Throbocytopenia and Thrombosis
  • Heparin-Associated Thrombosis (HAT)
  • Heparin-Dependent Antiplatelet Antibodies
  • Heparin Serotonin Release Assay
  • Heparin-Induced Thrombosis (HIT)
  • Platelet Aggregation Factor
  • Platelet Aggregation Factor, Heparin Dependent
  • SRA (Serotonin Release Assay)
  • Serotonin Release, Heparin-Induced
  • Serotonin Secretion Assay for HAT
  • BCSRA
  • Serotonin Releasing Assay
  • Heparin-Dependent Platelet Antibody (SRA) - Unfractionated Heparin
Laboratory
Sendout
Reference Laboratory
Mayo SRAU
Section
Special Testing
Specimen Requirements
Collection Instructions

Screen for Heparin Antibody Assay (HITAB) will be performed. If the Heparin Antibody Assay is negative the Serotonin Release Assay will be cancelled with a comment added to both test orders indicating the reason for cancellation.

Collect Serotonin Release Assay specimen in a red top tube; do not use SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate or freeze. Citrated or ACD plasma specimen is acceptable if serum is not available. Specify Lovenox® (LMWH) or Unfractionated Heparin (UFH).

Contraindications
This test is not indicated if the Heparin Antibody Assay (HITAB) is negative.
Normal Volume
1 mL serum AND full 2.7 mL tube or 1 mL plasma aliquot
Minimum Volume
1 mL serum AND full 1.8 mL tube or 1 mL plasma aliquot
Rejection Criteria
SST tube not acceptable.
Additional Information

Requests for the Serotonin Release Assay will be sent to the MLabs Coagulation Laboratory for confirmation of a positive screen for Heparin Antibody Assay (HITAB) at an additional charge prior to forwarding to Mayo Clinic Laboratories. If the Heparin Antibody Assay is negative the Serotonin Release Assay will be cancelled with a comment added to both test orders indicating the reason for cancellation. Test sent to Mayo Clinic Laboratories.

Billing
CPT Code
82542
Fee Code
AB054
NY State Approved
No