Test Overview
Test Methodology

Fluorescence In Situ Hybridization (FISH)

Test Usage

This test detects rearrangements involving the ROS1 gene region (6q22) via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded tissue specimens. FISH is performed using break-apart probes (Abbott Molecular) to qualitatively assess for the presence of ROS1 translocations that occur in non-small cell lung cancer. Patients with locally advanced or metastatic non-small cell lung cancer (NSCLC) that is positive for ROS1 rearrangement may respond to crizotinib therapy.

Reference Range *

Interpretive report provided.

Test Limitations

This test will detect rearrangements involving ROS1, however it will not identify the translocation partner.

Test Details
Days Set Up
Monday - Thursday
Analytic Time

3 - 10 days

Soft Order Code
MROS1
MiChart Code
ROS1 (6q22) Rearrangement by FISH
Synonyms
  • Crizotinib evaluation for Non-small cell lung cancer (NSCLC)
  • MROS1
Laboratory
Molecular Diagnostics
Section
Molecular Diagnostics
Specimen Requirements
Collection Instructions

A formalin-fixed, paraffin-embedded tissue block containing sufficient neoplastic cells is preferred. Unstained slides (3, 4-micron slides) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Normal Volume
Formalin-fixed, paraffin-embedded tissue
Additional Information

By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.

Billing
CPT Code
88377-TC
Fee Code
NA049
Pro Fee CPT
88377-26
NY State Approved
No