Test Overview

Test Methodology

Direct Immunofluorescence using flow cytometric techniques.

Test Usage

Evaluation of Rituximab therapy. A full lymphoid cell panel is run, including B and T cell quantitation and natural killer cell quantitation. Markers include CD3, CD4, CD8, CD16/56, CD19, CD20 and CD4:CD8 ratio (T4:T8 ratio). See http://www.pathology.med.umich.edu/handbook/Tables/Flow_Cytometry_Panel… for all Flow Cytometry Panels.

Reference Range*

[link text="RITUXAN reference range.docx" href="/static/apps/handbook/Tables/RITUXAN reference range.docx"]
[bold]*Pediatric Reference Ranges from Journal of Allergy and Clinical Immunology, vol. 112, No. 5, 11/2003 and studies performed at Michigan Medicine Flow Cytometry Laboratory. Absolute ranges are calculated utilizing absolute lymphocyte count ranges for the same age groups provided by the hematology laboratory.[/bold]

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Requires viable cells which have been stored at room temperature (18-22 degrees C) for

Test Details

Days Test Performed

Monday - Friday: 7:00am - 5:00pm
Saturday: 7:00am - 3:00pm

Analytic Time

24 - 48 hours

Soft Order Code


MiChart Code

Rituximab Therapy Profile, Flow Cytometry



Generic Flow Test
Markers CD3 CD4 CD8 CD16/56 CD19 CD20
Rituxan Therapy
Rituximab Treatment Profile
Lymphoma Treatment
Panel 7: Rituximab
Rituxan Panel Shadow

+ See More


Flow Cytometry

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Specimen Requirements

Offsite Collection Instructions

Collect specimen in a yellow top ACD A or B Vacutainer® tube.
Include a copy of the patient's complete blood count (CBC) and white cell differential drawn concurrently or within the previous 8 hours.
Send intact whole blood stored at room temperature within 72 hours of collection; do not refrigerate.

Rejection Criteria

Green top (lithium heparin) Vacutainer® tubes are not acceptable.

Normal Volume

7 - 10 mL ACD whole blood

Minimum Volume

3 mL ACD whole blood

Additional Information

Additional testing will be run at the discretion of the hematopathologist at an additional charge with interpretation if clinically indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. For consultation, contact the MLabs Client Services Center or Flow Cytometry Laboratory at 76-39420.

Billing Information

Fee Codes

CPT Code

86355 x2, 86357, 86359, 86360

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