Test Overview
Test Methodology

Direct Immunofluorescence using flow cytometric techniques.

Test Usage

Evaluation of Rituximab therapy. A full lymphoid cell panel is run, including B and T cell quantitation and natural killer cell quantitation. Markers include CD3, CD4, CD8, CD16/56, CD19, CD20 and CD4:CD8 ratio (T4:T8 ratio). See http://www.pathology.med.umich.edu/handbook/Tables/Flow_Cytometry_Panel… for all Flow Cytometry Panels.

Reference Range *

RITUXAN reference range.docx
*Pediatric Reference Ranges from Journal of Allergy and Clinical Immunology, vol. 112, No. 5, 11/2003 and studies performed at Michigan Medicine Flow Cytometry Laboratory. Absolute ranges are calculated utilizing absolute lymphocyte count ranges for the same age groups provided by the hematology laboratory.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Requires viable cells which have been stored at room temperature (18-22 degrees C) for

Test Details
Days Set Up
Monday - Friday: 7:00am - 5:00pm
Saturday: 7:00am - 3:00pm
Analytic Time

24 - 48 hours

Soft Order Code
MiChart Code
Rituximab Therapy Profile, Flow Cytometry
  • Generic Flow Test
  • Markers CD3 CD4 CD8 CD16/56 CD19 CD20
  • Rituxan Therapy
  • Rituximab Treatment Profile
  • Lymphoma Treatment
  • CD56
  • Panel 7: Rituximab
  • Rituxan Panel Shadow
Specimen Requirements
Collection Instructions

Collect specimen in a yellow top ACD A or B Vacutainer® tube.
Include a copy of the patient's complete blood count (CBC) and white cell differential drawn concurrently or within the previous 8 hours.
Send intact whole blood stored at room temperature within 72 hours of collection; do not refrigerate.

Alternate Specimen
Lavender top EDTA and Green top (sodium heparin) Vacutainer® tubes are acceptable but not preferred, and must be received within 12 hours of collection.
Normal Volume
7 - 10 mL ACD whole blood
Minimum Volume
3 mL ACD whole blood
Rejection Criteria
Green top (lithium heparin) Vacutainer® tubes are not acceptable.
Additional Information

Additional testing will be run at the discretion of the hematopathologist at an additional charge with interpretation if clinically indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. For consultation, contact the MLabs Client Services Center or Flow Cytometry Laboratory at 76-39420.

CPT Code
86355 x2, 86357, 86359, 86360
Fee Code
23043 x2, 23047, 23044, 23045