Test Overview
Test Methodology

Indirect Fluorescent Antibody (IFA)

Test Usage

Antigen specific IgG and IgM titers allow rapid diagnosis of infection by any of the spotted fever group of rickettsial agents. This group of agents include R. rickettsii (Rocky Mountain Spotted Fever) and R. akari (rickettsial pox), both seen in the continental United States.

Reference Range *

Screen: Not detected. Titer: <1:64.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Tuesday, Thursday
Analytic Time

1 - 5 days

Soft Order Code
MiChart Code
  • Febrile Agglutinins
  • Rocky Mountain Spotted Fever
  • Weil-Felix Agglutinins
  • Spotted Fever Group Antibody
  • Rickettsia rickettsii
  • Tick Borne Disease
Reference Laboratory
Quest Valencia 6419
Special Testing
Specimen Requirements
Collection Instructions

Collect blood in a red top or SST tube. Centrifuge, aliquot serum into plastic vial and refrigerate. EDTA (lavender top), heparinized (green top) or ACD (yellow top) plasma is also acceptable.

Normal Volume
1 mL serum
Minimum Volume
0.5 mL serum
Additional Information

If IgG or IgM antibodies are detected the appropriate titer will be performed at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. Test sent to Quest Diagnostics Nichols Institute of Valencia.

CPT Code
86757 x2
Reflex CPT
86757 per titer
32999-5 RMSF IgG, 33000-1 RMSF IgM