Test Overview
Test Methodology

Fluorescence In Situ Hybridization (FISH)

Test Usage

This test detects rearrangements involving the RET gene region (10q11) via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded tissue specimens. FISH is performed using break-apart probes (Abbott Molecular) to qualitatively assess for the presence of RET translocations that occur in non-small cell lung cancer (NSCLC) and thyroid carcinomas. Lung cancer patients with tumors harboring RET rearrangements may benefit from kinase inhibitor therapy.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

This test will detect rearrangements involving RET but will not identify the translocation partner. This test may not detect RET rearrangements in some cases due to the intrachromosomal proximity of some fusion partners.

Test Details
Days Set Up
Monday - Thursday
Analytic Time

3 - 10 days

Soft Order Code
MRET
MiChart Code
RET (10q11) Rearrangement by FISH
Synonyms
  • MRET
Laboratory
Molecular Diagnostics
Section
Molecular Diagnostics
Specimen Requirements
Collection Instructions

A formalin-fixed, paraffin-embedded tissue block containing sufficient neoplastic cells is preferred. Unstained slides (3, 4-micron slides) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Normal Volume
Formalin-fixed, paraffin-embedded tissue
Additional Information

By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.

Billing
CPT Code
88377-TC
Fee Code
NA048
Pro Fee CPT
88377-26
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