Test Overview
Test Methodology

Film Array multiplex Polymerase Chain Reaction (PCR)

Test Usage

Detection of respiratory viruses Adenovirus, Coronavirus 229E, Coronavirus NL63, Coronavirus OC43, Human Metapneumovirus, Influenza A, Influenza A subtype H1, Influenza A subtype H3, Influenza A subtype 2009 H1, Influenza B, Parainfluenza virus 1, Parainfluenza virus 2, Parainfluenza virus 3, Parainfluenza virus 4, Rhinovirus/Enterovirus, Respiratory Syncytial Virus (RSV), Bordetella pertussis, Chlamydophila pneumoniae, and Mycoplasma pneumoniae.

Reference Range *

Negative

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Due to the genetic similarity between Rhinovirus and Enterovirus, this test cannot reliably differentiate them. Similarly, the Coronavirus OC43 assay may cross-react with Coronavirus HKU1. B. pertussis results from this assay may not be concordant with the Bordetella PCR assay (test code PCRBP) that targets the multi-copy insertion sequence (IS481) due to differences in sensitivity and specificity. The detection of viral and bacterial nucleic acid is dependent upon proper specimen collection, handling, transportation, storage and preparation. Positive and negative predictive values are highly dependent on prevalence. A negative result does not exclude the possibility of viral or bacterial infection. Negative test results may occur from the presence of sequence variants in the region targeted by the assay, the presence of inhibitors, or an infection caused by an organism not detected by the panel. Test results may also be affected by concurrent antiviral/antibacterial therapy or levels of organism in the specimen that are below the limit of detection for the test. Positive results do not rule co-infection with other organisms. Viral and bacterial nucleic acids may persist in vivo independent of organism viability. Results of this test should not be used as the sole basis for diagnosis, treatment or other management decisions.

Test Details
Days Set Up
Daily
Analytic Time

1 day (6 hours for UM inpatients and ED).
Testing is performed 24/7 throughout the day with turnaround time dependent on staffing and the current load on the instrument.

Soft Order Code
RPAN
MiChart Code
Respiratory Virus Panel by PCR
Synonyms
  • RESPIRATORY VIRUSES; PCR
  • PCRIR
  • Influenza Viruses by PCR
  • Respiratory Viruses Panel
  • Human Metapneumovirus by PCR
  • Influenza A by PCR
  • RSV by PCR
  • Parainfluenza Viruses by PCR
  • Respiratory Syncytial Virus by PCR
  • Influenza B by PCR
  • Viral Respiratory Screen, Comprehensive
  • ERPAN
  • SFLA
  • SFLB
  • Parainfluenza 1, 2, 3, 4 Viruses by PCR
  • hMPV by PCR
  • RPAN
  • Coronavirus by PCR
  • Mycoplasma pneumoniae by PCR
  • Human Rhinovirus / Enterovirus by PCR
  • Chlamydophila pneumoniae by PCR
  • Adenovirus by PCR
  • Influenza A subtypes H1, H3, 2009 H1
  • Bordetella pertussis
Laboratory
Microbiology
Section
Microbiology
Test Updated
Update Type
Test Down or Delayed
Specimen Requirements
Collection

SWAB: Use flocked swab, dacron or rayon swab to obtain specimen and place into M4-RT transport medium. Refrigerate. BAL FLUID: Place in sterile cup and refrigerate. M6-RT or UTM transport media is also acceptable. Deliver all specimen types to the laboratory as soon as possible. The possibility for virus isolation decreases as the length of storage time increases. Many viral agents will survive at room temperature for several hours; however, for optimum recovery of viruses, please refrigerate; do not freeze.

Special Handling

For patients seen in the ER (CES or ESA) please order test code ERPAN instead of RPAN. Test code ERPAN will receive priority testing and is only approved for use by clinicians in the emergency department.

Rejection Criteria
specimens collected with a calcium alginate swab or wooden handled swab.
Normal Volume
1 nasopharyngeal swab; 2 mL BAL; 2 mL tracheal aspirate or suction
Storage Temperature
Refrigerate 2-8 C
Additional Information

Additional laboratory testing including viral and bacterial culture, immunofluorescence and radiography may be necessary to evaluate respiratory tract infections. Specimen will be held for a minimum of 2 weeks to allow additional testing to be added as needed.

Billing
CPT Code
0100U
Fee Code
LA024
LOINC
82159-5
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