Test Overview
Test Methodology

Rapid Fluorescent Focus Inhibition Test (RFFIT)

Test Usage

This assay is designed to measure anti-rabies glycoprotein antibodies induced by rabies vaccination only; it should not be used as a tool to assess natural exposure to rabies virus.

Reference Range *

Reportable range is 0.1 - 15.0 IU/mL. In humans a result of 0.5 IU/mL or higher is considered an acceptable response to rabies vaccination according to the World Health Organization (WHO) guidelines; see WHO and Advisory Committee on Immunization Practices documents for additional guidance.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday, Wednesday, Thursday
Analytic Time

3 - 5 weeks

Soft Order Code
MiChart Code
Rabies Antibody, Sendout
  • Rabies Antibody, ELISA
  • Rabies Antibody Titer Check (Endpoint)
Reference Laboratory
Mayo FRFIT (90330) (Kansas State University)
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in red top tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Post-vaccination samples should be collected 4-6 weeks from the date of vaccination.

Alternate Specimen
CSF specimens are acceptable. [5/09]
Normal Volume
2 mL serum
Minimum Volume
0.5 mL serum
Storage Temperature
Refrigerated (preferred): 14 days; Frozen: 60 days.
Additional Information

Test sent to Mayo Medical Laboratories; performed Kansas State University Rabies Laboratory.

CPT Code
Fee Code