Test Overview
Test Methodology

Rapid Fluorescent Focus Inhibition Test (RFFIT)

Test Usage

This assay is designed to measure anti-rabies glycoprotein antibodies induced by rabies vaccination only; it should not be used as a tool to assess natural exposure to rabies virus.

Reference Range *

Reportable range is 0.1 - 15.0 IU/mL. In humans a result of 0.5 IU/mL or higher is considered an acceptable response to rabies vaccination according to the World Health Organization (WHO) guidelines; see WHO and Advisory Committee on Immunization Practices documents for additional guidance.

Test Details
Days Set Up
Monday, Wednesday, Thursday
Analytic Time

3 - 5 weeks

Soft Order Code
RABAB
MiChart Code
Rabies Antibody, Sendout
Synonyms
  • RABAB
  • Rabies Antibody, ELISA
  • Rabies Antibody Titer Check (Endpoint)
Laboratory
Sendout
Reference Laboratory
Mayo FRFIT (90330) (Kansas State University)
Section
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in red top tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Post-vaccination samples should be collected 4-6 weeks from the date of vaccination.

Alternate Specimen
CSF specimens are acceptable. [5/09]
Normal Volume
2 mL serum
Minimum Volume
0.5 mL serum
Storage Temperature
Refrigerated (preferred): 14 days; Frozen: 60 days.
Additional Information

Test sent to Mayo Medical Laboratories; performed Kansas State University Rabies Laboratory.

Billing
CPT Code
86382
Fee Code
AA815
LOINC
5288-6
NY State Approved
No