Test Overview
Test Methodology

Direct Immunofluorescence using flow cytometric techniques

Test Usage

A full quantitative lymphoid cell panel is run, including B, T, and natural killer cell enumeration to aid in the diagnosis and monitoring of patients who may be affected by primary immunodeficiencies, acquired immunodeficiencies, drug therapies, or immune reconstitution following transplantation. Markers include CD3, CD4, CD8, CD16/56, CD19, CD20 and CD4:CD8 ratio (T4:T8 ratio).

Test Limitations

Requires viable cells which have been stored at room temperature (18-22 degrees C)

Test Details
Days Set Up
Monday - Friday: 8:00 am - 4:00 pm
Analytic Time

24 - 48 hours

Soft Order Code
QTBNK
MiChart Code
Quantitative T & B Lymphocyte Subset, QTBNK
Synonyms
  • Immunodeficiencies profile
  • Flow Cytometry
  • Rituximab therapy
  • Organ transplant monitoring
  • OKT3 Antigen
  • T-helper / T-suppressor ratio
  • Natural killer cell count
  • Markers CD3 CD4 CD8 CD1656 CD19 CD20
  • T and B cell subsets
  • T cell and B cell lymphocyte surface markers
  • CD4:CD8 (T4:T8) ratio
  • • Organ Transplant Monitoring
  • • Rituximab Treatment Profile
  • • Rituxan Therapy
  • • OKT3 Monitoring
  • • ATGAM Monitoring
  • • OKT3 Antigen
  • • Primary Immunodeficiency Profile
  • • Primary Immunodeficiencies Profile
  • • CD4:CD8 (T4:T8) Ratio
  • Markers CD3 CD4 CD8 CD16/56 CD19 CD20
  • • Inherited Immunodeficiency Profile
  • • Lymphocyte Surface Marker Assay
  • • Natural Killer Cell Count
  • • T Cell and B Cell Lymphocyte Surface Markers
  • • T and B Cell Subsets
  • • B and T Cell Subsets
  • • Acquired Immunodeficiencies Profile
  • • Acquired Immunodeficiency Profile
  • • Absolute T Cell Count
  • • AIDS (Acquired Immune Deficiency Syndrome) Profile
  • • Helper / Suppressor ratio
  • • T Cell subsets
  • • T-Helper / T-Suppressor ratio
Laboratory
Hematopathology
Section
Hematology
Specimen Requirements
Collection Instructions

<ul><li>Collect specimen in a Sodium Heparin Vacutainer® tube.
</li><li>Include a copy of the patient's complete blood count (CBC) and white cell differential drawn concurrently or within the previous 8 hours.
</li><li>Send intact whole blood stored at room temperature within 72 hours of collection; do not refrigerate.
</li></ul>

Alternate Specimen
Lavender top EDTA and ACD A or B Vacutainer® tubes are acceptable but not preferred, and must be received within 12 hours of collection.
Normal Volume
3-4 ml
Minimum Volume
1 mL whole blood drawn into a Sodium Heparin Vacutainer® tube
Rejection Criteria
Clotted samples cannot be tested. Green top (lithium heparin) Vacutainer® tubes are not acceptable.
Additional Information

Additional testing will be run at the discretion of the pathologist at an additional charge with interpretation if clinically indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. For consultation, contact the MLabs Client Services Center or the Clinical Core Laboratory at 936-6866

Billing
CPT Code
86355, 86357, 86359, 86360
Fee Code
23043, 23047, 23044, 23045