Test Overview

Test Methodology


Test Usage

Isolate, quantitate and identify aerobic organisms.

Reference Range*

No growth

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Quantitation of organisms isolated may not be accurate if < 0.005 gram of tissue is received. Quantitative culture detects aerobic organisms and yeast; other organisms may not be detected.

Test Details

Days Test Performed

Monday - Friday
Specimens received on weekends before noon on Sunday will be processed on Sunday.

Analytic Time

Preliminary results are reported at 24 hours and quantitative results are reported within 48 hours. Identifications and antibiotic susceptibilities (if requested) may take longer. Negative cultures are reported after 48 hours.

Soft Order Code


MiChart Code

Deep Tissue Culture, Quantitative, Burn Lab



Culture, Deep Tissue or Bone Quantitation, Aerobe
Biopsy, Quantitation Culture, Aerobe only
Biopsy Quantitation, Aerobic Culture
Aerobic Bacterial Culture, Deep Tissue or Bone Quantitation
Aerobic Culture, Deep Tissue or Bone Quantitation
Quantitation Deep Tissue or Bone Culture, Aerobic
Surgical Specimen Quantitation, Aerobic Culture
Tissue (Deep), Quantitation Culture, Aerobe only
Bone, Quantitation Culture, Aerobe only

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Specimen Requirements

Offsite Collection Instructions

Aseptically prepare biopsy site before taking biopsy. The portion of the surgical specimen submitted for this test should be separated from the portion submitted for other testing. For optimum recovery, send immediately. A small gauze moistened with sterile saline may be added to prevent desiccation. If specimen must be stored, refrigerate immediately after specimen collection. May be refrigerated up to 48 hours; do not freeze. Indicate specimen source, collection date/time, current antibiotic therapy, and clinical diagnosis. If an unacceptable specimen is received, the client will be notified and another specimen will be requested before disposal of the specimen.

Rejection Criteria

Specimens received in preservative such as Formalin are not acceptable.
Specimens that are too small to be weighed will be reported as “quantity not sufficient”.
Specimens not been properly stored such as not refrigerated or older than 3 days will not be processed unless a new specimen can not be obtained and the physician requests processing.
Specimens received in containers that are grossly contaminated during collection or from having inadequately tightened lids may be rejected if the technologist is unable to access tissue safely.


100ml Plastic Specimen Container

Normal Volume

0.005 - 0.2 gram of involved tissue or 3 - 4 mm punch biopsy

Minimum Volume

0.005 gram of tissue

Additional Information

Test includes quantitative culture, Gram stain, and identification of organisms. Test includes identification and susceptibility testing of potentially pathogenic organism(s) at an additional charge unless specifically declined. Quantitative culture results are reported within the range of 10-1,000,000,000 organisms per gram of tissue. Isolates from deep tissue or bone cultures will be sent to the Microbiology Laboratory for antibiotic susceptibilities. Topical sensitivities are not performed unless requested.

Billing Information

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CPT Code


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