Test Overview
Test Methodology

High-Pressure Liquid Chromatography (HPLC) with Fluorescence Detection

Test Usage

Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria.

Reference Range *

Free Protoporphyrin: <20 mcg/dL packed cells; Zinc-Complexed Protoporphyrin: <60 mcg/dL packed cells.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Wednesday -Friday
Analytic Time

2 - 6 days

Soft Order Code
MPPFE
MiChart Code
Protoporphyrins, Fractionation, Whole Blood
Synonyms
  • Erythrocyte Protoporphrin
  • Erythrocyte Protoporphyrins, Fractionation
  • FEP
  • FEP (Free Erythrocyte Protoporphyrins)
  • Free Erythrocyte Protoporphyrins (FEP)
  • Free Erythrocyte Protoporphyrins (FEP)
  • PROTOPORPHYRINS, FRACT., RBC
  • PPFE FREE
  • PPFE ZINC
  • PPFE-D
  • FREE PROTOPORPHYRIN
  • ZINC-COMPLEXED PROTOPORPHYRIN
  • Protoporphyrins, Fractionation, RBC
  • PPFE
  • PPFB
  • Protoporphyrins, Fract, BLOOD
Laboratory
Sendout
Reference Laboratory
Mayo PPFE (8739)
Section
Special Testing
Specimen Requirements
Collection Instructions

Patient should abstain from alcohol for 24 hours and be off medications for 1 week prior to specimen collection. If it is clinically inappropriate to discontinue medications, forward a list of medications with the specimen. Collect blood in a green top tube from a fasting patient (12 - 14 hour fast). Place on wet ice immediately. Refrigerate and send intact whole blood specimen within 24 hours of collection.

Special Handling

Fasting specimen. Place on ice immediately.

Normal Volume
5 mL heparinized whole blood
Minimum Volume
3 mL heparinized whole blood
Additional Information

Includes separate quantitation of zinc protoporphyrin and free protoporphyrin. Test sent to Mayo Medical Laboratories.

Billing
CPT Code
82542
Fee Code
AA221
LOINC
Z166-9
NY State Approved
No
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