Test Overview
Test Methodology

High-Pressure Liquid Chromatography (HPLC) with Fluorescence Detection

Test Usage

Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria.

Reference Range *

Free Protoporphyrin: <20 mcg/dL packed cells; Zinc-Complexed Protoporphyrin: <60 mcg/dL packed cells.

Test Details
Days Set Up
Monday - Wednesday -Friday
Analytic Time

2 - 6 days

Soft Order Code
MPPFE
MiChart Code
Protoporphyrins, Fractionation, Whole Blood
Synonyms
  • Erythrocyte Protoporphrin
  • Erythrocyte Protoporphyrins, Fractionation
  • FEP
  • FEP (Free Erythrocyte Protoporphyrins)
  • Free Erythrocyte Protoporphyrins (FEP)
  • Free Erythrocyte Protoporphyrins (FEP)
  • PROTOPORPHYRINS, FRACT., RBC
  • PPFE FREE
  • PPFE ZINC
  • PPFE-D
  • FREE PROTOPORPHYRIN
  • ZINC-COMPLEXED PROTOPORPHYRIN
  • Protoporphyrins, Fractionation, RBC
  • PPFE
  • PPFB
  • Protoporphyrins, Fract, BLOOD
Laboratory
Sendout
Reference Laboratory
Mayo PPFE (8739)
Section
Special Testing
Specimen Requirements
Collection Instructions

Patient should abstain from alcohol for 24 hours and be off medications for 1 week prior to specimen collection. If it is clinically inappropriate to discontinue medications, forward a list of medications with the specimen. Collect blood in a green top tube from a fasting patient (12 - 14 hour fast). Place on wet ice immediately. Refrigerate and send intact whole blood specimen within 24 hours of collection.

Special Handling

Fasting specimen. Place on ice immediately.

Alternate Specimen
Green top with lithium heparin, navy top with heparin
Normal Volume
5 mL heparinized whole blood
Minimum Volume
3 mL heparinized whole blood
Storage Temperature
Refrigerated only
Additional Information

Includes separate quantitation of zinc protoporphyrin and free protoporphyrin. Test sent to Mayo Medical Laboratories.

Billing
CPT Code
82542
Fee Code
AA221
LOINC
Z166-9
NY State Approved
No