Test Overview
Test Methodology

High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection

Test Usage

Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria.

Reference Range *

Free Protoporphyrin: <20 mcg/dL packed cells; Zinc-Complexed Protoporphyrin: <60 mcg/dL packed cells.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday, Wednesday, Friday
Analytic Time

2 - 4 days

Soft Order Code
MiChart Code
Protoporphyrins, Fractionation, RBC
  • Protoporphyrins, Fractionation, Washed Erythrocytes
Reference Laboratory
Mayo PPFWE (31891)
Special Testing
Specimen Requirements

Patient should abstain from alcohol for 24 hours and be off medications for 1 week prior to specimen collection. If it is clinically inappropriate to discontinue medications, forward a list of medications with the specimen. Collect blood in a green top tube from a fasting patient (12 - 14 hour fast). Place on wet ice immediately. Process as follows:
a. Transfer entire specimen to a 12-mL graduated centrifuge tube.
b. Centrifuge specimen for 10 minutes at 2,000 rpm.
c. Record volume of packed cells and the total volume of the specimen.
d. Discard supernatant plasma.
e. Wash packed erythrocytes 2 times by resuspension of at least an equal amount of cold 0.9% saline, mix, and centrifuge for 5 minutes at 2,000 rpm, discarding supernatant after each washing.
f. Resuspend packed cells to the original total volume with 0.9% saline. Invert specimen gently to mix.
g. Transfer washed erythrocytes into a plastic vial and freeze.
Volume of packed cells and total volume of erythrocyte suspension (red cells + saline) are required and must be sent with specimen.

Special Handling

Fasting specimen. Place on ice immediately following collection. See below for processing instuctions.

Normal Volume
3 mL washed erythrocyte suspension
Minimum Volume
1 mL washed erythrocyte suspension
Additional Information

Includes separate quantitation of zinc protoporphyrin and free protoporphyrin. Test sent to Mayo Medical Laboratories.

CPT Code
Fee Code