Test Overview
Test Methodology

Allelic Discrimination, Polymerase Chain Reaction (PCR)

Test Usage

Prothrombin (Factor II) is the precursor of the serine protease thrombin, a key enzyme in the process of hemostasis and thrombosis, that exhibits procoagulant, anticoagulant and antifibrinolytic activities. One nucleotide change in the prothrombin gene – F2 c.*97G>A (rs1799963; g.46761055G>A; NC_000011.9; NM_000506.3; commonly referred to as 20210G>A), is associated with elevated prothrombin levels, an increased risk of venous thromboembolism and recurrent thromboembolism. Other genetic as well as environmental factors may also influence the risk of thrombosis. Genetic counseling may be indicated.

Reference Range *

Interpretive report provided.

Test Details
Days Set Up
*Due to COVID-19 testing the analytical time for this assay has changed to 2 - 14 days. This assay will now be performed once every two weeks instead of weekly.
Analytic Time

2 - 14 days

Soft Order Code
PROMT
MiChart Code
Prothrombin (Factor II) c.*97G>A (20210)
Synonyms
  • Factor II Mutation Assay
  • PROTH
  • Prothrombin 20210 by DNA Analysis
  • Prothrombin Promoter Mutation
  • PROINV
  • PROINVS
  • PROIN
  • Prothrombin 20210 Mutation
  • MLABEL
  • PROTHROMBIN 20210
  • Prothrombin Progenitor Gene Test
Laboratory
Molecular Diagnostics
Section
Molecular Diagnostics
Specimen Requirements
Collection Instructions

Collect blood in a lavender top tube. Refrigerate and send intact blood specimen within 48 hours of collection.

Special Handling

To reduce inappropriate thrombophilia testing in the inpatient population this test has been removed from the inpatient test catalog. If there is a specific clinical need for this test on an inpatient please contact the Coagulation Laboratory.

Alternate Specimen
The preferred specimen is whole blood collected in EDTA (lavender top). However, ACD (yellow top) and heparin (green top) may also be accepted.
Contraindications
In the absence of specific information regarding advances in the knowledge of mutation characteristics for a particular disorder, the current literature indicates that genetic tests for inherited disease need only be conducted once per lifetime.
Lavender Top Tube
Normal Volume
5 mL EDTA (lavender) whole blood
Additional Information

By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.

Billing
CPT Code
81240
Fee Code
21682
Pro Fee CPT
G0452-26
LOINC
24475-6
NY State Approved
No