Test Overview
Test Methodology

Optical Clot-Based

Test Usage

Second order testing for diagnosis of congenital or acquired protein S deficiency, for example, as an adjunct to initial testing based on results of free protein S antigen assay (PSAGF). Evaluating patients with a history of venous thromboembolism.

Reference Range *

Male: 65 - 150%, Female <50 years: 50 - 150, Female > or =50 years: 65 - 150%. Newborn infants have normal or near-normal free protein S antigen (> or =50%), although total protein S antigen is usually below the adult reference range. There are insufficient data concerning protein S activity in normal neonates, infants, and children; but normal or near-normal activity (> or =50%) probably is present by age 3 to 6 months.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday
Analytic Time

1 - 4 days

Soft Order Code
MSFX
MiChart Code
Protein S Activity
Synonyms
    Laboratory
    Sendout
    Reference Laboratory
    Mayo SFX
    Section
    Special Testing
    Specimen Requirements
    Collection Instructions

    Collect specimen in a blue top (citrate 3.2%) tube. Mix by inversion. Specimen should arrive at lab within 3 hours of collection; transport at room temperature. Alternatively, centrifuge, aliquot plasma into a plastic vial, and freeze the specimen within 4 hours of collection. Transport frozen specimen on dry ice. Collection of the blood through lines that have been previously flushed with heparin should be avoided. If the blood must be drawn through a VAD (vascular access device), the line should be flushed with 5 mL of saline and the first 5 mL of blood or six dead space volumes of the VAD discarded.

    Special Handling

    Note that the Protein S Antigen, Free (PSAGF) assay is the recommended test for screening for inherited Protein S Deficiency.

    Contraindications
    This test is not indicated in patients receiving warfarin (coumadin) therapy.
    Normal Volume
    Full 2.7mL tube or 1mL plasma aliquot
    Minimum Volume
    Full 1.8mL tube or 1mL plasma aliquot
    Storage Temperature
    Room temperature for whole blood or frozen aliquot of citrated plasma.
    Rejection Criteria
    Specimens will be rejected if not properly filled, clotted, grossly hemolyzed, or contaminated with heparin.
    Additional Information

    Test sent to Mayo Clinic Laboratories.

    Billing
    CPT Code
    85306
    Fee Code
    22196
    LOINC
    27822-6
    NY State Approved
    No