Test Overview
Test Methodology

High-Performance Liquid Chromatography (HPLC) with Fluorometric Detection

Test Usage

Screening test during symptomatic periods for acute intermittent porphyria, hereditary coproporphyria, and variegate porphyria (additional confirmatory testing is required to establish a diagnosis). Screening test to begin assessment for congenital erythropoietic porphyria and porphyria cutanea tarda (additional confirmatory testing is required to establish a diagnosis).

Reference Range *

UROPORPHYRINS (octacarboxyl): < or =30 nmol/24 hours. HEPTACARBOXYLPORPHYRINS: < or =9 nmol/24 hours. HEXACARBOXYLPORPHYRINS: < or =8 nmol/24 hours. PENTACARBOXYLPORPHYRINS: < or =10 nmol/24 hours. COPROPORPHYRINS (tetracarboxyl): Males: < or =230 nmol/24 hours; Females: < or =168 nmol/24 hours. PORPHOBILINOGEN: < or =2.2 mcmol/24 hours.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday
Analytic Time

2 - 3 days

Soft Order Code
MiChart Code
Porphyrins, Quant, 24 Hour Urine
  • Coproporphyrins
  • Porphyrins, Fractionation, Urine
  • Uroporphyrins
  • UROQ
Reference Laboratory
Mayo PQNU (8562)
Special Testing
Specimen Requirements

Patient should abstain from alcohol for 24 hours prior to, as well as during, collection. Collect 24 hour urine specimen. Add 5 grams of Sodium Carbonate (1 gram for pediatric patients) to container prior to start of collection; do not use Sodium Bicarbonate. The container should be refrigerated and protected from light as much as possible during collection. Following collection, mix well, measure 24 hour urine volume, aliquot 50 mL into a plastic urine container and freeze. Protect specimen from light. Record total 24 hour urine volume and collection dates/times on request form.

Special Handling

Protect specimen from light.

Normal Volume
50 mL aliquot of 24 hour urine collection
Minimum Volume
20 mL aliquot of 24 hour urine collection
Additional Information

Test includes quantitation of coproporphyrins, uroporphyrins, intermediate porphyrins (heptacarboxyl, hexacarboxyl, pentacarboxyl), and porphobilinogen. Test sent to Mayo Medical Laboratories.

CPT Code
84120, 84110
Fee Code