ROM Plus - in-vitro qualitative immunochromatography
Performed at authorized locations by point of care operators. The ROM PLUS is used to detect the presence of amniotic fluid in the vaginal canal. High concentrations of AFP and PP12 are seen in Amniotic fluid, which contrasts the vaginal mucosa. Normal rupturing of the amniotic sac due to uterine contractions is a biological process that occurs just before child birth (ROM). Spontaneous rupturing without uterine contractions is referred to as premature rupture of membrane (PROM) If the amniotic sac ruptures before 37 weeks of pregnancy it is considered as preterm premature rupture of the membraned (PPROM). PROM and PPROM may cause the following sequelae; excessive hemorrhaging (placenta and uterus separation), blood clots, cord prolapse, infection, still birth and many other life threatening conditions that can affect both the infant and mother. Early and accurate detection of the PROM or PPROM increases the chances for the patient to have a positive outcome with fewer complications.
Negative (indicating no premature rupture of membranes, PROM)
Note: Normal positive results are seen in patients who are at full term pregnancy and the rupturing of the amniotic sac is brought on by uterine contractions.
•The ROM Plus is a qualitative assay and is reported as "Negative" and "Positive".
•Excessive vaginal bloody discharge may cause a false positive result
5 - 20 minutes
1. Remove all the contents from the ROM Plus foil pouch. Inside will be a test cassette with a timer, sterile vaginal swab and a vial containing buffered solution. The vaginal swab is sterile and should remain so until the actual collection of the patient sample. Only opening the swab's packaging half way leaving the wooden portion exposed will keep the swab tip sterile and also make accessible when ready to collect.
2. To collect, remove the vaginal swab by grabbing the wooden section. Without touching an other surface, insert the polyester tip of the swab 2- 3 inches in to the vagina. Allow the swab to remain in vagina for a minimum of 15 seconds before withdrawing.
For more test specific information, please refer to the test procedure found on the Michigan Medicine POC website: https://www.pathology.med.umich.edu/point-of-care.