Test Overview
Test Methodology

Flow Cytometric Immunophenotyping

Test Usage

Screening or confirming the diagnosis of Paroxysmal Nocturnal Hemoglobinuria (PNH). See http://www.pathology.med.umich.edu/handbook/Tables/Flow_Cytometry_Panel… for all Flow Cytometry Panels.

Reference Range *

RED BLOOD CELLS:

PNH RBC-Partial Antigen loss: 0.00-0.99%

PNH RBC-Complete Antigen loss: 0.00-0.01%

PNH Granulocytes: 0.00-0.01%

PNH Monocytes: 0.00-0.05%

Test Limitations

Requires an adequate number of viable cells for analysis. Improper collection or transport may result in decreased cell viability and cancellation of testing.

Test Details
Days Set Up
Monday - Friday
Analytic Time

1 - 2 days

Soft Order Code
PLINK
MiChart Code
Paroxysmal Nocturnal Hemoglobinuria (PNH) Profile, Flow Cytometry
Synonyms
  • INULIN TEST FOR PNH
  • Acid Hemolysin
  • GPI-Linked Antigens
  • Ham's Test
  • Paroxysmal Nocturnal Hemoglobinuria (PNH)
  • CD14
  • CD24
  • FLAER
  • PNH
  • INULIN
  • Panel 8: Paroxysmal Nocturnal Hemoglobinuria (PNH)
  • Pi-Linked Antigen, Blood
  • PNH MARKER PANEL
  • CD33
  • CD45
  • CD59
  • Hams Test
  • PNHS
  • PNH Marker Panel Shadow
Laboratory
Sendout
Reference Laboratory
Mayo PLINK
Section
Special Testing
Specimen Requirements
Collection Instructions

The following information is required on a Hematopathology Consult requisition:
[list order =Y]
Relevant clinical history
Clinical or morphological findings and suspicions
Date and time of collection
Phone number and after hours pager number for ordering physician
[/list]
<ul><li>
Collect blood in a yellow top ACD solution A or B Vacutainer® tube.<li>
Send intact specimen at room temperature within 12 hours of collection.<li>
Do not refrigerate or freeze.
</ul>

Rejection Criteria
Green top (sodium or lithium heparin) tubes are not acceptable. Samples with gross hemolysis are not acceptable.
Normal Volume
2.6 - 10 mL ACD (solution A or B) whole blood
Minimum Volume
1 mL ACD (solution A or B) whole blood
Additional Information

Test includes WBC panel (CD14, CD15, CD16, CD24, CD33, CD45, FLAER) and RBC panel (CD235a, CD59). Test performed by Mayo Clinic Laboratories.

Billing
CPT Code
88184 x2, 88185 x7
Fee Code
AA895 x2, AA896 x7
Reflex Fee Code
21583
NY State Approved
No