Test Overview
Flow Cytometric Immunophenotyping
Screening or confirming the diagnosis of Paroxysmal Nocturnal Hemoglobinuria (PNH). See http://www.pathology.med.umich.edu/handbook/Tables/Flow_Cytometry_Panel… for all Flow Cytometry Panels.
RED BLOOD CELLS:
PNH RBC-Partial Antigen loss: 0.00-0.99%
PNH RBC-Complete Antigen loss: 0.00-0.01%
PNH Granulocytes: 0.00-0.01%
PNH Monocytes: 0.00-0.05%
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Requires an adequate number of viable cells for analysis. Improper collection or transport may result in decreased cell viability and cancellation of testing.
Test Details
2 - 4 days
- Acid Hemolysin
- GPI-Linked Antigens
- Ham's Test
- Paroxysmal Nocturnal Hemoglobinuria (PNH)
- CD14
- CD24
- FLAER
- PNH
- Panel 8: Paroxysmal Nocturnal Hemoglobinuria (PNH)
- Pi-Linked Antigen, Blood
- CD33
- CD45
- CD59
Specimen Requirements
The following information is required on a Hematopathology Consult requisition:
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Relevant clinical history
Clinical or morphological findings and suspicions
Date and time of collection
Phone number and after hours pager number for ordering physician
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Collect blood in a yellow top ACD solution A or B Vacutainer® tube.<li>(EDTA is acceptable)
Send intact specimen specimen at room temp to arrive at Mayo within 72 hours of collection.<li>
Do not freeze.
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Additional Information
Test includes WBC panel (CD14, CD15, CD16, CD24, CD33, CD45, FLAER) and RBC panel (CD235a, CD59). Test performed by Mayo Clinic Laboratories.