Test Overview
Test Methodology

Enzyme Linked Immunosorbent Assay (ELISA)

Test Usage

Approximately 75-80% of patients have serum autoantibodies to the M-type phospholipase A2 receptor (PLAR2). In contrast, less than 10% of patients with secondary membranous nephropathy, other forms of glomerulonephritis, as well as healthy individuals are positive for autoantibody to the PLA2R antibody. An increase, decrease or disappearance generally precedes a change in the clinical status, thus the antibody titer has a predictive value with respect to clinical remission, relapse, or risk assessment after kidney transplantation. A test for colocalization of PLA2R and IgG4 of kidney tissue is recommended in seronegative samples to confirm or exclude a diagnosis of PLA2R antibody-mediated MGN.

Reference Range *

Positive: >20 U/mL Borderline: 14 to 20 U/mL Negative: <14 U/mL.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Analytic Time

7 - 10 days

Soft Order Code
  • PLA2R
  • Anti-PLA2R
  • Anti-Phospholipase A2 Receptor Antibody
  • Phospholipase A2 Receptor Autoantibody
Reference Laboratory
Cincinnati Children's Hospital 8193540
Special Testing
Specimen Requirements

Collect specimen in an SST tube. Centrifuge, aliquot serum into a screw capped leak-proof container, and freeze.

Yellow Top Tube
Normal Volume
0.5 mL serum
Minimum Volume
0.2 mL serum
Additional Information

Test sent to Cincinnati Children’s Hospital.

CPT Code
Fee Code