Test Overview
Test Methodology

Enzyme Linked Immunosorbent Assay (ELISA)

Test Usage

Approximately 75-80% of patients have serum autoantibodies to the M-type phospholipase A2 receptor (PLAR2). In contrast, less than 10% of patients with secondary membranous nephropathy, other forms of glomerulonephritis, as well as healthy individuals are positive for autoantibody to the PLA2R antibody. An increase, decrease or disappearance generally precedes a change in the clinical status, thus the antibody titer has a predictive value with respect to clinical remission, relapse, or risk assessment after kidney transplantation. A test for colocalization of PLA2R and IgG4 of kidney tissue is recommended in seronegative samples to confirm or exclude a diagnosis of PLA2R antibody-mediated MGN.

Reference Range *

Positive: >20 U/mL Borderline: 14 to 20 U/mL Negative: <14 U/mL.

Test Details
Analytic Time

7 - 10 days

Soft Order Code
  • PLA2R
  • Anti-PLA2R
  • Anti-Phospholipase A2 Receptor Antibody
  • Phospholipase A2 Receptor Autoantibody
Reference Laboratory
Cincinnati Children's Hospital 8193540
Special Testing
Specimen Requirements

Collect specimen in an SST tube. Centrifuge, aliquot serum into a screw capped leak-proof container, and freeze.

Yellow Top Tube
Normal Volume
0.5 mL serum
Minimum Volume
0.2 mL serum
Additional Information

Test sent to Cincinnati Children’s Hospital.

CPT Code
Fee Code
NY State Approved