Test Overview
Test Methodology

Flow Cytometry

Test Limitations

This flow cytometry assay is intended to be used as a screening test. Screening tests are not 100% sensitive nor specific, and a normal result should not preclude molecular sequencing if a patient's clinical presentation suggests that the probability of a diagnosis is high.

Test Details
Days Set Up
Monday - Friday
Analytic Time

2 days

Soft Order Code
PFORN
MiChart Code
Perforin/Granzyme B
Synonyms
    Laboratory
    Sendout
    Reference Laboratory
    Cincinnati Children's Hospital 2903500
    Section
    Special Testing
    Specimen Requirements
    Collection

    Specimens are accepted Monday through Thursday between 12:00 noon and 4:00 pm only and must be received by the performing laboratory within 24 hours of collection. Collect specimen in lavender top tube. Send intact whole blood at room temperature. Do not refrigerate or freeze.

    Special Handling

    Specimen must be received Monday - Thursday between 12:00 noon and 4:00 pm.

    Alternate Specimen
    Green top (sodium heparin)
    Normal Volume
    3 mL EDTA whole blood
    Minimum Volume
    1 mL EDTA whole blood
    Storage Temperature
    Room temperature. DO NOT REFRIGERATE OR FREEZE.
    Additional Information

    Test includes intracellular expression of Perforin and Granzyme B in NK cells, CD8+ cells, and NKT cells. Test sent to Cincinnati Children's Hospital.

    Billing
    CPT Code
    88184, 88185 x4
    Fee Code
    AA714, AA715 x4
    NY State Approved
    No