Test Overview
Test Methodology

Flow Cytometry

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

This flow cytometry assay is intended to be used as a screening test. Screening tests are not 100% sensitive nor specific, and a normal result should not preclude molecular sequencing if a patient's clinical presentation suggests that the probability of a diagnosis is high.

Test Details
Days Set Up
Monday - Friday
Analytic Time

2 days

Soft Order Code
PFORN
MiChart Code
Perforin/Granzyme B
Laboratory
Sendout
Reference Laboratory
Cincinnati Children's Hospital 2903500
Section
Special Testing
Specimen Requirements
Collection

Specimens are accepted Monday through Thursday between 12:00 noon and 4:00 pm only and must be received by the performing laboratory within 24 hours of collection. Collect specimen in lavender top tube. Send intact whole blood at room temperature. Do not refrigerate or freeze.

Special Handling

Specimen must be received Monday - Thursday between 12:00 noon and 4:00 pm.

Alternate Specimen
Green top (sodium heparin)
Lavender Top Tube
Normal Volume
3 mL EDTA whole blood
Minimum Volume
1 mL EDTA whole blood
Storage Temperature
Room temperature. DO NOT REFRIGERATE OR FREEZE.
Additional Information

Test includes intracellular expression of Perforin and Granzyme B in NK cells, CD8+ cells, and NKT cells. Test sent to Cincinnati Children's Hospital.

Billing
CPT Code
88184, 88185 x4
Fee Code
AA714, AA715 x4
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