Test Overview
Flow Cytometry Immunophenotyping
Screening or confirming the diagnosis of Paroxysmal Nocturnal Hemoglobinuria (PNH). See http://www.pathology.med.umich.edu/handbook/Tables/Flow_Cytometry_Panel… for all Flow Cytometry Panels.
RED BLOOD CELLS: PNH RBC-Partial Antigen Loss: 0.00-0.02%
PNH RBC-Complete Antigen Loss: 0.00-0.01%
PNH Granulocytes: 0.00-0.01%
PNH Monocytes: 0.00-0.1%
*Reference ranges may change over time. Please refer to the original patient report when evaluating results.*
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Requires an adequate number of viable cells for analysis. Improper collection or transport may result in decreased cell viability and cancellation of testing.
Test Details
Specimen Requirements
Offsite Collection:
The following information is required on a Hematopathology Consult requisition:
1. Relevant clinical history
2. Clinical or morphological findings and suspicions
3. Date and time of collection
4. Phone number and after hours pager number for ordering physician
* Collect blood in a yellow top ACD A or B Vacutainer® tube. (EDTA is acceptable)
* Send intact specimen at room temperature within 12 hours of collection. Specimen should arrive at the Flow Cytometry Lab within 72 hours of collection.
* Do not freeze.
Clotted peripheral blood specimens are unacceptable.
Samples with gross hemolysis are unacceptable.
Additional Information
Test includes WBC panel (CD14, CD15, CD24, CD45, CD64, FLAER) and RBC panel (CD235a, CD59). Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation. Additional markers will be run if clinically indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. For consultation, contact the MLabs Client Services Center or Flow Cytometry Laboratory at 76-39420.