Label a PreservCyt container with patient last name, first name and registration number (or other second unique identifier). Gently insert a cytobrush into the anus to a distance of 2-3 cm, ensuring sampling of the ano-rectal junction; rotate the brush or swab 360 degrees while gently pulling back and forth. Place brush directly into PreservCyt, rotate the brush in the solution 10 times while also pushing the brush against the wall of the container. Swirl the brush vigorously in the solution to further release cells. Submit with an appropriately completed requisition, including pertinent patient history. Store the specimen at room temperature. If PreservCyt is not available, label a glass slide with patient's last name, first name and registration number (or other second unique identifier) using a graphite pencil (do not use ink, wax or crayon). Gently roll brush on glass slide to cover the area of a dime and fix each smear IMMEDIATELY with spray fixative. Allow spray-fixed slides to dry completely and place into a slide holder. Submit with an appropriately completed requisition, including pertinent patient history.