5-12 days
Test Updated:
N-RAS Mutation Detection
NRAS
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Test Overview
Next-Generation Sequencing
Activating mutations in the NRAS gene occur in approximately 2-3% of colorectal carcinomas, 20% of melanomas, and a variety of other human cancers. NRAS mutations are predominantly single nucleotide substitutions, occurring most frequently within exons 2 and 3 at codons 12, 13, and 61. These NRAS mutations are associated with poor clinical response to epidermal growth factor receptor (EGFR) targeted therapies in patients with colorectal cancer. This DNA test is performed by targeted next-generation sequencing to detect mutations within sequenced regions of NRAS exons 2, 3, and 4 (NM_002524.4; Hg19 chr1:115285690-115258774, chr1:115256505-115256584, chr1:115252191-115252305) including codons 12, 13, and 61. Specimens should contain an adequate proportion of neoplastic cells (>20%) in the area to be extracted to ensure mutation detection.
Interpretive report provided.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Specimen Requirements
For formalin-fixed, paraffin-embedded tissue, a block containing an area with a high percentage of neoplastic cells (for micro-/macro-dissection) is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature. A Diff-Quik or Papanicolaou stained aspirate smear (preferable containing a high percentage and overall amount of neoplastic cells) is also acceptable. Store at room temperature..