Days Set Up
Monday - Friday
Analytic Time

2 - 7 days

MiChart Code
MPL Mutation
Soft Order Code

Test Updated:


W515A Mutation
W515K Mutation
W515L Mutation
MPL Mutation Detection
S505N Mutation

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Find a Requisition

All specimens should be accompanied by a requisition.

Submitting Specimens

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MLabs provides all the supplies necessary for the collection of specimens.

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Test Overview

Test Methodology

Multiplex Polymerase Chain Reaction (PCR) with Capillary Electrophoresis

Test Usage

MPL gene mutations occur in cases of primary myelofibrosis (PMF) and essential thrombocythemia (ET) at a frequency of ~10% and 3% respectively. Testing for MPL mutations may aid in the diagnosis of these myeloproliferative neoplasms. MPL mutations are usually found in cases that test negative for the JAK2 V617F mutation, although a small number of patients have been reported with both mutations. This test will qualitatively detect MPL mutations (W515L, W515K, W515A, and S505N) in peripheral blood or bone marrow with a sensitivity down to 5% mutant allele.

Reference Range

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Offsite

Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice.

Normal Volume
5 mL EDTA (lavender) whole blood; 3 mL EDTA (lavender) bone marrow; Fresh/frozen cells in RPMI. Extracted DNA is also acceptable if extracted in a CLIA certified laboratory.
Minimum Volume

Billing Information

CPT Code
Pro Fee Code

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.