Test Overview
Test Methodology

Flow Cytometry; multi-fluorochrome

Test Usage

This test is typically performed on bone marrow aspirate, day 29 post therapy. In patients with B-cell acute lymphoblastic leukemia (ALL), treatment response is evaluated with minimal residual disease (MRD) assays.

Reference Range *

Pathologist interpretation of results provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm; Saturday 8:00am-12:00noon
Analytic Time

8 - 48 hrs

Soft Order Code
MRDBM
MiChart Code
Minimal Residual Disease for B-ALL; Bone Marrow
Laboratory
Flow Cytometry
Section
Flow Cytometry
Specimen Requirements
Collection Instructions

The following information is required on a Hematopathology Consult requisition:
<ol><li>Relevant clinical history
</li><li>Clinical or morphological findings and suspicions
</li><li>Specimen source
</li><li>Date and time of collection
</li><li>Phone number and after hours pager number for ordering physician
</li></ul></ol>

<strong>BONE MARROW ASPIRATE:</strong>
<ul><li>Add 1 - 2 ml of first pull bone marrow aspirate to a green top sodium heparin Vacutainer® tube. Send intact specimen at room temperature within 12 hours of collection.
</li><li>Include 4 unstained aspirate smears
</li><li>Include an unstained peripheral blood smear
</li><li>Include a copy of the patient's most recent White Blood Cell and platelet counts, and peripheral blood differential.
</li></ul>

Special Handling

Samples received in heparin at room temperature.
Sample should be less than 48 hours old when set up. Specimens may be processed after 48
hours and will be processed up to 72 hours and reported with a comment regarding
specimen age.

Contraindications
This test should NOT replace send out testing for MRD-B ALL anti-CD19 testing at Johns Hopkins
Normal Volume
<strong>BONE MARROW ASPIRATE:</strong> 1-2 ml
Minimum Volume
<strong>BONE MARROW ASPIRATE:</strong> 1 ml
Storage Temperature
Samples received in heparin at room temperature.
Sample should be less than 48 hours old when set up. Specimens may be processed after 48
hours and will be processed up to 72 hours and reported with a comment regarding
specimen age.
Additional Information

Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation. Additonal markers will be run if clinically indicated. Measurement of TdT may be an adjunct to the diagnosis of some leukemias and lymphoblastic lymphomas and will be run at the discretion of the hematopathologist at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. For consultation, contact the MLabs Client Services Center or Flow Cytometry Laboratory at 76-39420.

Billing
CPT Code
88184, 88185 x11, 88188
Fee Code
23033, 23041 x11
Pro Fee Code
88188
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