Test Overview
Test Methodology

The FilmArray Meningitis/Encephalitis (ME) Panel is a multiplex PCR capable of simultaneous detection and identification of multiple bacterial, viral, and yeast nucleic acids directly from cerebrospinal fluid (CSF) specimens obtained via lumbar puncture from individuals with signs and/or symptoms of meningitis and/or encephalitis. The following organisms are identified using the FilmArray ME Panel:
Bacteria:
Escherichia coli K1
Haemophilus influenzae
Listeria monocytogenes
Neisseria meningitidis (encapsulated)
Streptococcus agalactiae
Streptococcus pneumoniae
Viruses:
Cytomegalovirus
Enterovirus
Herpes simplex virus 1
Herpes simplex virus 2
Human herpesvirus 6
Human parechovirus
Varicella zoster virus
Yeast:
Cryptococcus neoformans/gattii

Test Usage

Meningitis/Encephalitis Panel (MEPAN) is available for use via a new reflex testing algorithm for cerebrospinal fluid (CSF) specimens. When “CSF Analysis with Reflex to MEPAN” is selected, MEPAN add-on testing will be automatically performed on CSF specimens that meet any of the following criteria:
• WBC > 5 cells/mm³
• Protein > 50 mg/dL
• Glucose < 40 mg/dL

The MEPAN can also be ordered directly with appropriate infectious disease approval or when ordered as indicated by the febrile infant guidelines (https://michmed-clinical.policystat.com/policy/12261468/latest/).

Test Limitations

Dedicated testing for HSV (HSV DNA by PCR, Qualitative, CSF) and Cryptococcus (Cryptococcus Antigen, CSF) are more sensitive than the same targets on the MEPAN and thus should be ordered prior to the MEPAN if clinically indicated.
The FilmArray ME Panel is indicated as an aid in the diagnosis of specific agents of meningitis and/or encephalitis and results are meant to be used in conjunction with other clinical, epidemiological, and laboratory data. Results are not intended to be used as the sole basis for diagnosis, treatment, or other patient management decisions. Positive results do not rule out co-infection with organisms not included in the FilmArray ME Panel. The agent detected may not be the definite cause of the disease. Negative results do not preclude central nervous system (CNS) infection. The FilmArray ME Panel is not intended for testing of specimens collected from indwelling CNS medical devices.

Test Details
Days Set Up
Monday - Sunday
Analytic Time

3 hours

Soft Order Code
MEPAN
Synonyms
  • Haemophilus influenzae
Laboratory
Microbiology
Section
Microbiology
Specimen Requirements
Collection

CSF specimens should be collected via lumbar puncture, and should not be centrifuged.

Normal Volume
2 mL
Minimum Volume
0.2 mL
Storage Temperature
CSF may be stored for up to 24 hours at room temperature (approximately 23 C), or refrigerated (approximately 4 C ) for up to 7 days.
Billing
CPT Code
87483
Fee Code
LA020
NY State Approved
No