Test Overview
Test Methodology

The coding exon or a part of the coding exon containing the MECP2 familial mutation is amplified using specific primers and bidirectionally sequenced using a fluorescent method.

Test Usage

MECP2 targeted sequencing can be performed on a patient with a known familial mutation. If MECP2 sequencing that detected the familial mutation was not performed at MMGL Molecular Genetics Lab, then please send a copy of the clinical laboratory report that documents the familial mutation.

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

This analysis will not identify mutations in the regulatory elements, deep intronic regions or coding regions of MECP2 that are not covered by this targeted test. This assay may not detect large deletion or duplication mutations and balanced translocations involving MECP2 gene. Variant classification reflects the current state of scientific understanding at the time of test completion. As new scientific data becomes available, the interpretation and classification of variants identified in this assay may change. Test interpretation may be impacted by the presence of a hematologic malignancy or an allogenic bone marrow transplant.

Test Details
Days Set Up
Monday - Friday
Analytic Time

28 days

Soft Order Code
MECF
MiChart Code
MECP2 Targeted Sequencing, Familial
Synonyms
  • MECF
  • Rett Syndrome
  • Autism / Intellectual Disability
Laboratory
MMGL
Section
MMGL Molecular Genetics
Specimen Requirements
Collection Instructions

Collect blood specimen in a EDTA lavender top tube. Send it within 24 hours if stored at room temperature or within 5 days if stored refrigerated.

Fill out a MLabs Molecular test requisition (or place an e-order if applicable):
https://mlabs.umich.edu/media/166
Fill out a Clinical History Form for Insurance Prior Authorization (include all required documentation indicated at the bottom of this form):
https://mlabs.umich.edu/sites/default/files/2020-08/file/mlab11618clini…
Fill out a UMHS Request and Consent for Genetic Testing form:
https://mlabs.umich.edu/sites/default/files/2023-04/file/germline-conse…
Michigan State law requires the ordering provider to obtaining informed consent from the patient prior to prognostic or predictive genetic testing. Contact a MLabs Client Services Center at 800-862-7284 to request paper copies or to setup a client account.

Alternate Specimen
Cheek swab kit (Oragene OCD-100), follow collection instructions.
Saliva kit (Oragene OGD-510), follow collection instructions.
Normal Volume
5 mL whole blood
Minimum Volume
1 mL whole blood (0.5 mL infants)
Storage Temperature
Room temperature up to 24 hours or 2-8°C up to 5 days for blood
2-36°C for saliva
Rejection Criteria
Hemolyzed blood sample. Fresh tissue (POC) or paraffin embedded specimens are not acceptable.
Additional Information

Most insurance carriers require prior authorization for genetic testing. Testing will not begin until insurance prior authorization is received by the laboratory or it is confirmed that prior authorization is not required. The ordering health care provider can obtain the prior authorization or request the laboratory to submit it. To obtain BCN prior authorization call Joint Venture Hospital Laboratories (JVHL) at 800-445-4979; for all other insurances, contact the plan directly. By ordering this test the clinician acknowledges that informed consent, https://mlabs.umich.edu/sites/default/files/2023-04/file/germline-conse…, has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes medical geneticist interpretation of results billed as a separate additional charge. This test is not available without interpretation.

Billing
CPT Code
81303
Fee Code
DA017
Pro Fee CPT
G0452-26