3 - 10 days
Test Updated:
12q15
MMDM2
Chromosome 12 centromere (CEP12)
Liposarcoma
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Test Overview
Fluorescence In Situ Hybridization (FISH)
This test detects amplification of the MDM2 gene region (12q15) via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded tissue specimens. FISH is performed using Vysis (Abbott Molecular, Inc.) probes to the MDM2 locus (12q15) and the chromosome 12 centromere (CEP12). MDM2 amplification is present in most cases of atypical lipomatous tumor/well-differentiated liposarcoma and most cases of dedifferentiated liposarcoma. MDM2 amplification is not seen in benign lipomatous tumors. Testing for MDM2 amplification may aid in the diagnosis and classification of these tumors.
Interpretive report provided.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Specimen Requirements
A formalin-fixed, paraffin-embedded tissue block containing sufficient neoplastic cells is preferred. Unstained slides (3, 4-micron slides) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.