Days Set Up
Monday - Friday
Analytic Time

2 - 7 days

MiChart Code
KIT D816V Mutation
Soft Order Code

Test Updated:


Systemic Mastocytosis
KIT c.2447A>T (D816V) Mutation
KIT (D816V) Mutation Shadow
c-KIT Mutation
KIT Asp816Val Mutation Analysis

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Test Overview

Test Methodology

Allele-specific Polymerase Chain Reaction (PCR) with capillary electrophoresis detection.

Test Usage

This test is used for qualitative detection of the KIT c.2447A>T (D816V) mutation found in most adults (>80%) with systemic mastocytosis. Detection of the KIT D816V mutation can aid in diagnosis of systemic mastocytosis and guide choice of therapy since it is associated with resistance to imatinib mesylate. The KIT D816V mutation also occurs in some cases of acute myelogenous leukemia and seminoma. This test is not intended to detect minimal residual disease.

Reference Range

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Offsite

Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fixed cytogenetic cell suspensions or pellets in Carnoy’s fixative. Refrigerate and send. For formalin-fixed, paraffin-embedded tissue, a block containing the high percentage of neoplastic cells is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Lavender Top Tube
Normal Volume
5 mL EDTA (lavender) whole blood; 3 mL EDTA (lavender) bone marrow; Fresh/frozen cells in RPMI; Formalin-fixed, paraffin-embedded tissue; Fixed cytogenetic cell suspensions or pellets in Carnoy’s fixative. Extracted DNA is also acceptable if extracted in a CLIA certified laboratory.
Minimum Volume

Billing Information

CPT Code
Pro Fee Code

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.