Days Set Up
Monday - Friday
Analytic Time

2 - 7 days

MiChart Code
JAK2 V617F Mutation
Soft Order Code

Test Updated:


JAK2 c.1849G>T (V617F) Mutation
Tyrosine kinase Mutation
Janus kinase 2 gene Mutation
JAK2 (V617F) Mutation Analysis
JAK2 (V617F) Mutation Shadow
Polycythemia Vera
Essential Thrombocythemia
Idiopathic Myelofibrosis
LAP Score

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Test Overview

Test Methodology

Polymerase Chain Reaction (PCR)

Test Usage

Detection of the JAK2 c.1849G>T (V617F) mutation in myeloproliferative neoplasms: polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF). This test is indicated for evaluation of patients with unexplained and sustained elevation of red blood cell or platelet counts, splenomegaly or bone marrow fibrosis of undetermined causation, and patients in whom a diagnosis of a chronic myeloproliferative disorder is a consideration. The JAK2 V617F mutation is detectable in approximately 95% PV, 55% ET, and 65% PMF patients. A negative result does not rule out a diagnosis of PV, ET, or PMF.

Reference Range

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Offsite

Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh tissue (preferably 0.5cm3, sent in RPMI) and fresh aspirates or body fluids are acceptable. Refrigerate and send, preferably within 24 hours. Frozen tissue specimens – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice.

Lavender Top Tube
Normal Volume
5 mL EDTA (lavender) whole blood; 3 mL EDTA (lavender) bone marrow; Fresh/frozen tissue and fresh aspirates or body fluids; Fresh/frozen cells in RPMI. Extracted DNA is also acceptable if extracted in a CLIA certified laboratory.
Minimum Volume

Billing Information

CPT Code
Pro Fee Code

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.