Test Overview
Test Methodology

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Test Usage

Verifying systemic absorption of orally administered itraconazole. This test is indicated in patients with life-threatening fungal infections and in patients considered at risk for poor absorption or rapid clearance.

Reference Range *

Itraconazole (trough): >0.5 mcg/mL (localized infection); >1.0 mcg/mL (systemic infection). Hydroxyitraconazole: No therapeutic range established; activity and serum concentration are similar to parent drug.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday through Saturday
Analytic Time

1 - 3 days

Soft Order Code
MiChart Code
Itraconazole Level
  • Sporanox
  • Itraconazole, S/P
Reference Laboratory
Mayo ITCON (81247)
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in a red top tube; do not use SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. For maximum clinical significance, usual sampling times are Peak: 30 minutes after the end of an I.V. infusion (60 minutes after I.M. or oral administration) or Trough: immediately before next dose is given.

Normal Volume
1 mL serum
Minimum Volume
0.2 mL serum
Rejection Criteria
SST tube not acceptable.
Additional Information

Test sent to Mayo Medical Laboratories.

CPT Code
Fee Code