8 - 48 hrs
B and T Cell Typing for Lymphocytic Leukemias
Leukemia / Lymphoma Immunophenotyping
Lymphocyte Typing for Lymphoproliferative Disorders
Lymphocytic Leukemias, Acute and Chronic Subtyping
T and B Cell Typing for Lymphocytic Leukemias
Chronic Lymphocytic Leukemia (CLL)
Mixed Linage Acute Leukemia
Acute Lymphocytic Leukemia (ALL)
Acute Myelogenous Leukemia (AML)
Leukemia/Lymphoma, Uncertain Subtype
Plasma Cell Panel (PCL)
ACUTE MARKER PANEL
NONACUTE MARKER PANEL
Prolymphocytic Leukemia (PLL)
Mantle Cell Lymphoma/Leukemia (MCL)
Hairy Cell Leukemia (HCL)
Diffuse Large B-Cell Lymphoma (DLBCL)
Leukemia/Lymphoma Acute Marker Panel
Leukemia/Lymphoma Nonacute Marker Panel
Leukemia/Lymphoma Combo Marker Panel
Panel 3: Plasma Cell / Multiple Myeloma
Panel 1: Stem Cell Neoplasm / Acute Leukemia
Panel 2: Mature Lymphoid Leukemia / Non-Hodgkin Lymphoma
Panel 4: Comprehensive Leukemia / Lymphoma
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Direct immunofluorescence using flow cytometric techniques.
Confirmation of diagnosis and immunophenotype in acute and chronic leukemias and lymphomas. See http://www.pathology.med.umich.edu/handbook/Tables/Flow_Cytometry_Panel… for all Flow Cytometry Panels.
Pathologist interpretation of results provided.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
The following information is required on a Hematopathology Consult requisition:
Relevant clinical history
Clinical or morphological findings and suspicions
Date and time of collection
Phone number and after hours pager number for ordering physician
[bold]BONE MARROW ASPIRATE:[/bold]
Add 1 - 2 ml of first pull bone marrow aspirate to a green top sodium heparin Vacutainer® tube. Send intact specimen at room temperature within 12 hours of collection.
Include 4 unstained aspirate smears
Include an unstained peripheral blood smear
Include a copy of the patient's most recent White Blood Cell and platelet counts, and peripheral blood differential.
[bold]BODY FLUID and CSF:[/bold]
Add body fluid (e.g., CSF, pleural, peritoneal) to a clean, leak proof sterile container and send at room temperature within 12 hours of collection.
Include a a copy of the body fluid cell count and differential.
An original cytospin preparation (preferably unstained) should be included when possible with CSF specimens for correlative morphological evaluation.
[bold]BONE MARROW CORE BIOPSY AND FNA:[/bold]
Submerge in RPMI tissue culture medium (for optimal cell viability) or sterile saline and transport at room temperature within 12 hours of collection.
Mince and submerge in RPMI tissue culture medium (for optimal cell viability) or sterile saline, refrigerate, and transport refrigerated within 12 hours of collection.
[bold]BONE MARROW ASPIRATE:[/bold] 1-2 ml
[bold]BODY FLUIDS:[/bold] 20-50 ml
[bold]CSF:[/bold] 5-10 ml
[bold]BONE MARROW ASPIRATE:[/bold] 1 ml
[bold]BODY FLUIDS:[/bold] 10 ml*
[bold]CSF:[/bold] 1 ml*
* The volume of Body Fluids and CSF required is dependent on the cellularity of the specimen.