Test Overview
Test Methodology

Direct immunofluorescence using flow cytometric techniques.

Test Usage

Confirmation of diagnosis and immunophenotype in acute and chronic leukemias and lymphomas. See http://www.pathology.med.umich.edu/handbook/Tables/Flow_Cytometry_Panel… for all Flow Cytometry Panels.

Reference Range *

Pathologist interpretation of results provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Requires an adequate number of viable cells for analysis. When cell counts drop below 5 cells/cmm and

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm; Saturday 8:00am-4:30pm
Analytic Time

8 - 48 hrs

Soft Order Code
MiChart Code
Leukemia/Lymphoma Immunophenotyping; Non-Blood, Flow Cytometry
  • B and T Cell Typing for Lymphocytic Leukemias
  • Leukemia / Lymphoma Immunophenotyping
  • Lymphocyte Typing for Lymphoproliferative Disorders
  • Lymphocytic Leukemias, Acute and Chronic Subtyping
  • T and B Cell Typing for Lymphocytic Leukemias
  • Leukemias, Acute
  • Leukemias, Chronic
  • Chronic Lymphocytic Leukemia (CLL)
  • Mixed Linage Acute Leukemia
  • Acute Lymphocytic Leukemia (ALL)
  • Acute Myelogenous Leukemia (AML)
  • Leukemia/Lymphoma, Uncertain Subtype
  • Plasma Cell Panel (PCL)
  • Leukemia/Lymphoma Workup
  • Prolymphocytic Leukemia (PLL)
  • Mantle Cell Lymphoma/Leukemia (MCL)
  • Hairy Cell Leukemia (HCL)
  • Non-Hodgkin's Lymphoma
  • Follicular Lymphoma
  • Diffuse Large B-Cell Lymphoma (DLBCL)
  • Leukemia/Lymphoma Acute Marker Panel
  • Leukemia/Lymphoma Nonacute Marker Panel
  • Leukemia/Lymphoma Combo Marker Panel
  • Panel 3: Plasma Cell / Multiple Myeloma
  • Panel 1: Stem Cell Neoplasm / Acute Leukemia
  • Panel 2: Mature Lymphoid Leukemia / Non-Hodgkin Lymphoma
  • Panel 4: Comprehensive Leukemia / Lymphoma
  • Multiple Myeloma
  • Sezary Syndrome
  • T-Cell Leukemia
  • Cutaneous T-Cell Lymphoma Panel
  • Leukemia/Lymphoma Derm Marker Panel
Flow Cytometry
Flow Cytometry
Specimen Requirements
Collection Instructions

The following information is required on a Hematopathology Consult requisition:
Relevant clinical history
Clinical or morphological findings and suspicions
Specimen source
Date and time of collection
Phone number and after hours pager number for ordering physician

Add 1 - 2 ml of first pull bone marrow aspirate to a green top sodium heparin Vacutainer® tube.<li> Send intact specimen at room temperature within 12 hours of collection.<li>
Include 4 unstained aspirate smears
Include an unstained peripheral blood smear
Include a copy of the patient's most recent White Blood Cell and platelet counts, and peripheral blood differential.<li>
<b>BODY FLUID and CSF: </b>
Add body fluid (e.g., CSF, pleural, peritoneal) to a clean, leak proof sterile container and transport at room temperature within 12 hours of collection.<li>
Include a a copy of the body fluid cell count and differential.<li>
An original cytospin preparation (preferably unstained) should be included when possible with CSF specimens for correlative morphological evaluation.
Submerge in tissue culture medium such as RPMI (preferred) or equivalent, and transport at room temperature within 12 hours of collection.<li>
Mince and submerge in tissue culture medium such as RPMI (preferred) or equivalent, and transport at room temperature within 12 hours of collection.<li>

Normal Volume
<b>BONE MARROW ASPIRATE: </b> 1-2 ml
<b>BODY FLUIDS: </b> 20-50 ml
<b>CSF: </b> 5-10 ml
Minimum Volume
<b>BODY FLUIDS: </b> 10 ml*
<b>CSF: </b> 1 ml*
* The volume of Body Fluids and CSF required is dependent on the cellularity of the specimen.
Rejection Criteria
Green top with lithium heparin is unacceptable.
Specimens in formalin are unacceptable.
Additional Information

Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation. Additonal markers will be run if clinically indicated. Measurement of TdT may be an adjunct to the diagnosis of some leukemias and lymphoblastic lymphomas and will be run at the discretion of the hematopathologist at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. For consultation, contact the MLabs Client Services Center or Flow Cytometry Laboratory at 76-39420.

CPT Code
ACUTE: 88184, 88185 x23, 88189; NONACUTE: 88184, 88185 x13, 88188
Fee Code
ACUTE: 23033, 23041 x23; NONACUTE: 23033, 23041 x13
Pro Fee Code
ACUTE: 88189.1, NONACUTE: 88188.3