Test Overview
Test Methodology

Direct Immunofluorescence using flow cytometric techniques.

Test Usage

Evaluation of primary immunodeficiencies. A full lymphoid cell panel is run, including B and T cell quantitation and natural killer cell quantitation. Markers include CD3, CD4, CD8, CD16/56, CD19, and CD4:CD8 ratio (T4:T8 ratio).

Reference Range *

<a text="IMM reference ranges.docx" href="/static/apps/handbook/Tables/IMM%20reference%20ranges.docx">
<b>*Pediatric Reference Ranges from Journal of Allergy and Clinical Immunology, vol. 112, No. 5, 11/2003 and studies performed at Michigan Medicine Flow Cytometry Laboratory. Absolute ranges are calculated utilizing absolute lymphocyte count ranges for the same age groups provided by the hematology laboratory. </b>

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm
Analytic Time

24 - 48 hours

Soft Order Code
IMM
MiChart Code
Primary immune def. T,B,NK quantitation (CD3,4,8,19,16/56) Flow Cytometry I
Synonyms
  • FX FINAL
  • PRELIM
  • FLOW ADD
  • CD4:CD8 (T4:T8) Ratio
  • FC CAN
  • Primary Immunodeficiencies Profile
  • T&B Cell Lymphocyte Surface Markers
  • B and T Cell Subsets
  • Markers CD3 CD4 CD8 CD16/56 CD19
  • Inherited Immunodeficiency Profile
  • Lymphocyte Surface Marker Assay
  • Natural Killer Cell Count
  • Primary Immunodeficiency Profile
  • T Cell and B Cell Lymphocyte Surface Markers
  • IMM
  • IMMS
  • IMMUNODEFICIENCY PANEL
  • T and B Cell Subsets
  • FC CANCEL
  • FLOW CYTOMETRY ADDENDUM REPORT
  • FLOW FINAL
  • FLOW FINAL
  • FLOW PRELIMINARY
  • Primary Immunodef. Panel
  • FLABEL
  • Generic Flow Test
  • CD56
Laboratory
Flow Cytometry
Section
Flow Cytometry
Specimen Requirements
Collection Instructions

Date and time of collection is required on a flow cytometry requisition.
<ul><li>
Collect blood in ACD A or B Vacutainer® tube and transport at room temperature within 12 hours of collection.
Include a copy of the patient's concurrently drawn White Blood Cell and Platelet counts, and peripheral blood differential.
</ul>

Alternate Specimen
Green sodium heparin or lavender EDTA Vacutainer® tubes are acceptable but not preferred (must be received within 12 hours of collection). EDTA specimens greater than 30 hrs old cannot be processed.
Rejection Criteria
Green top tubes containing lithium heparin are not acceptable. Clotted specimens will be rejected
Normal Volume
Whole blood: 7-10 ml
Minimum Volume
Whole blood: 1 ml added to an ACD A or B tube
Additional Information

Additional testing will be run at the discretion of the hematopathologist at an additional charge with interpretation if clinically indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. For consultation, contact the MLabs Client Services Center or Flow Cytometry Laboratory at 76-39420.

Billing
CPT Code
86355, 86357, 86359, 86360
Fee Code
23043, 23047, 23044, 23045
LOINC
Z502-5
Resources