24 - 48 hours
CD4:CD8 (T4:T8) Ratio
Primary Immunodeficiencies Profile
T&B Cell Lymphocyte Surface Markers
B and T Cell Subsets
Markers CD3 CD4 CD8 CD16/56 CD19
Inherited Immunodeficiency Profile
Lymphocyte Surface Marker Assay
Natural Killer Cell Count
Primary Immunodeficiency Profile
T Cell and B Cell Lymphocyte Surface Markers
T and B Cell Subsets
FLOW CYTOMETRY ADDENDUM REPORT
Primary Immunodef. Panel
Generic Flow Test
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Direct Immunofluorescence using flow cytometric techniques.
Evaluation of primary immunodeficiencies. A full lymphoid cell panel is run, including B and T cell quantitation and natural killer cell quantitation. Markers include CD3, CD4, CD8, CD16/56, CD19, and CD4:CD8 ratio (T4:T8 ratio).
[link text="IMM reference ranges.docx" href="/static/apps/handbook/Tables/IMM%20reference%20ranges.docx"]
[bold]*Pediatric Reference Ranges from Journal of Allergy and Clinical Immunology, vol. 112, No. 5, 11/2003 and studies performed at Michigan Medicine Flow Cytometry Laboratory. Absolute ranges are calculated utilizing absolute lymphocyte count ranges for the same age groups provided by the hematology laboratory.[/bold]
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Date and time of collection is required on a flow cytometry requisition.
Collect blood in ACD A or B Vacutainer® tube and transport at room temperature within 12 hours of collection.
Include a copy of the patient's concurrently drawn White Blood Cell and Platelet counts, and peripheral blood differential.