Test Overview
Test Methodology

Direct Immunofluorescence using Flow Cytometric techniques.

Test Usage

Evaluation of acquired immunodeficiencies. A partial T-cell panel is run including CD3, CD4, CD8, and CD4:CD8 ratio (T4:T8 ratio).

Reference Range *

<a text="I3 reference ranges.docx" href="/static/apps/handbook/Tables/I3 reference ranges.docx">
<b>*Pediatric Reference Ranges from Journal of Allergy and Clinical Immunology, vol. 112, No. 5, 11/2003 and studies performed at Michigan Medicine Flow Cytometry Laboratory. Absolute ranges are calculated utilizing absolute lymphocyte count ranges for the same age groups provided by the hematology laboratory. </b>

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm;
Saturday- 8:00am - 12:00 noon
Analytic Time

24 - 48 hours

Soft Order Code
I3
MiChart Code
CD4 count/T-cell Subset Quantitation (CD3,4,8) Flow Cytometry I3
Synonyms
  • Acquired Immunodeficiencies Profile
  • PRELIM
  • Absolute T Cell Count
  • Acquired Immunodeficiency Profile
  • AIDS (Acquired Immune Deficiency Syndrome) Profile
  • Markers CD3 CD4 CD8
  • CD4:CD8 (T4:T8) Ratio
  • Helper / Suppressor ratio
  • T Cell subsets
  • T-Helper / T-Suppressor ratio
  • FX FINAL
  • FLOW ADD
  • FC CAN
  • I3
  • I3S
  • ACQUIRED IMMUNODEF. PANEL
  • FC CANCEL
  • FLOW CYTOMETRY ADDENDUM REPORT
  • FLOW FINAL
  • FLOW FINAL
  • FLOW PRELIMINARY
  • Acquired Immunodef. Panel
  • FLABEL
  • Generic Flow Test
  • T Cells CD3, CD4, and CD8 Count
Laboratory
Flow Cytometry
Section
Flow Cytometry
Specimen Requirements
Collection Instructions

Date and time of collection is required on a flow cytometry requisition.
<ul><li>
Collect blood in ACD A or B Vacutainer® tube and transport at room temperature within 12 hours of collection.
Include a copy of the patient's concurrently drawn White Blood Cell and Platelet counts, and peripheral blood differential.
</ul>

Alternate Specimen
Green sodium heparin or lavender EDTA Vacutainer® tubes are acceptable but not preferred (must be received within 12 hours of collection). EDTA specimens greater than 30 hrs old cannot be processed.
Rejection Criteria
Green top tubes containing lithium heparin are not acceptable. Clotted specimens will be rejected..
Normal Volume
Whole blood: 7-10 ml
Minimum Volume
Whole blood: 1 ml added to an ACD A or B tube
Additional Information

Additional testing will be run at the discretion of the hematopathologist at an additional charge with interpretation if clinically indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. For consultation, contact the MLabs Client Services Center or Flow Cytometry Laboratory at 76-39420.

Billing
CPT Code
86360, 86359
Fee Code
23045, 23044
LOINC
17146-2
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