Test Overview
Test Methodology

Fluorescence In Situ Hybridization (FISH)

Test Usage

This test detects the IGH::MYC t(8;14)(q24;q32) rearrangement using fluorescence in situ hybridization (FISH). FISH is performed with fluorescent probes directed at IGH (14q32), MYC (8q24) and the centromere of chromosome 8. The IGH::MYC t(8;14)(q24;q32) rearrangement occurs in most Burkitt lymphomas and a subset of large B-cell lymphomas including high-grade B-cell lymphoma with MYC and BCL2 rearrangements. Importantly, this test may identify MYC rearrangements not detected by MYC break-apart FISH and may, therefore, be used in conjunction with the MYC (8q24) Rearrangement by FISH test.

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

A negative result does not exclude a MYC rearrangement involving translocation partners other than IGH. This test may fail to detect a translocation in a sample with a low neoplastic burden.

Test Details
Days Set Up
Monday – Thursday
Analytic Time

3 – 10 days

Soft Order Code
MiChart Code
Molecular Diagnostics
Molecular Diagnostics
Specimen Requirements
Collection Instructions

A formalin-fixed, paraffin-embedded tissue block (containing sufficient neoplastic cells) is preferred. Unstained slides (3 slides cut at 4-microns) with associated H&E-stained slide are also acceptable. Decalcified tissue or tissues with other fixatives will be accepted and the assay attempted; however, these specimens may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Normal Volume
Formalin-fixed, paraffin-embedded tissue
Additional Information

By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation

CPT Code
Fee Code
Pro Fee CPT
NY State Approved