3 - 10 days
IGH/BCL2 TRANSLOCATION ASSAY
IGH/BCL2, t(14;18) Shadow
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Real-Time Polymerase Chain Reaction (PCR)
This test qualitatively detects IGH/BCL2, t(14;18) translocations involving the mbr, mcr, and icr breakpoint regions in peripheral blood, bone marrow, and fresh, frozen, or paraffin-embedded tissues. The t(14;18) translocation is the most frequent recurrent cytogenetic abnormality in follicular lymphoma, occurring in up to 90% of cases. The t(14;18) may also be identified in up to 30% of cases of de novo diffuse large B-cell lymphoma. The translocation joins the immunoglobulin heavy chain (IGH) locus on chromosome 14 and the BCL2 proto-oncogene on chromosome 18. The chromosome 14 breakpoints occur within the joining region (JH) of IGH, while the chromosome 18 breakpoints cluster within several distinct regions. These include the major (mbr), minor (mcr), and intermediate (icr) breakpoint cluster regions, which collectively account for approximately 70-80% of t(14;18) associated lymphomas.
Interpretive report provided.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh tissue (preferably 0.5cm3, sent in RPMI) and fresh aspirates or body fluids are acceptable. Refrigerate and send, preferably within 24 hours. Frozen tissue specimens – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. For formalin-fixed, paraffin-embedded tissue, a tissue block is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.