Fluorescence In Situ Hybridization (FISH)
This test detects the IGH/BCL2 t(14;18)(q32;q21) translocation via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded tissue specimens. FISH is performed using a dual-color dual-fusion probe set (Abbott Molecular) to qualitatively assess for the presence of the IGH/BCL2 translocation that occurs in most follicular lymphomas (FL) and a subset of diffuse large B-cell lymphomas (DLBCL). Testing for the IGH/BCL2 translocation may aid in the diagnosis of these tumors.
Interpretive report provided.
This test will only detect the IGH/BCL2 translocation and will not detect rare variant BCL2 translocations. In addition, this test may fail to detect a translocation in a sample with a low neoplastic burden.
- IHG/BCL2 t(14;18) by FISH
- t(14;18) Translocation
A formalin-fixed, paraffin-embedded tissue block containing sufficient neoplastic cells is preferred. Unstained slides (3, 4-micron slides) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.
By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.