Update Type: Miscellaneous
Test Updated: 01/30/2026
Test Overview
Test Methodology

Polymerase Chain Reaction (PCR) and Next-Generation Sequencing

Test Usage

Providing prognostic information in patients with newly diagnosed B-cell chronic lymphocytic leukemia

Reference Range *

See report

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday, Wednesday, Friday
Analytic Time

Up to two weeks

Soft Order Code
BCLL
Synonyms
    Laboratory
    Sendout
    Reference Laboratory
    Mayo BCLL
    Section
    Special Testing
    Specimen Requirements
    Collection Instructions

    Collect specimen in EDTA or ACD tube. Invert several times to mix. Send to Specimen Processing refrigerated

    Normal Volume

    4 mL Blood or 2 mL bone marrow; Extracted DNA from blood or bone marrow- 50mcl at 20ng/mcl concentration- label as extracted DNA

    Minimum Volume

    1 ml blood or 1 mL bone marrow; Extracted DNA - 50mcl at 20ng/mcl

    Storage Temperature
    Blood and bone marrow- refrigerated preferred, ambient acceptable
    DNA- frozen preferred, refrigerated acceptable
    Additional Information

    Test performed by Mayo Clinic Laboratories

    Regions of PIK3CA Covered

    Exon Codons Hg19 Start Hg19 Stop Transcript
    2 23 - 62 chr3:178,916,683 chr3:178,916,793 NM_006218.2
    2 75 - 114 chr3:178,916,848 chr3:178,916,953 NM_006218.2
    5 315 - 351 chr3:178,921,848 chr3:178,921,570 NM_006218.2
    6 354 - 379 chr3:178,922,288 chr3:178,922,365 NM_006218.2
    8 418 - 434 chr3:178,927,971 chr3:178,928,022 NM_006218.2
    8 449 - 468 chr3:178,928,070 chr3:178,928,129 NM_006218.2
    10 532 - 554 chr3:178,936,053 chr3:178,936,125 NM_006218.2
    14 692 - 729 chr3:178,938,832 chr3:178,938,948 NM_006218.2
    19 898 - 924 chr3:178,947,819 chr3:178,947,896 NM_006218.2
    21 1017 - 1051 chr3:178,951,997 chr3:178,952,097 NM_006218.2
    Billing
    CPT Code
    81263
    Fee Code
    AA503
    NY State Approved
    No